Skip to main content

Table 4 Summary of the three experimental repeats of the RDM analysis

From: Genome-wide polysomal analysis of a yeast strain with mutated ribosomal protein S9

   First Repeata Second Repeat Third Repeat
Gene (cleavage site) Resulting fragment Wild-type rps9-Δ188 Wild-type rps9-Δ188 Wild-type rps9-Δ188
YHB1 (cleavage at the middle of the ORF)b 5' half 13.7 14.3 13.5 15.2 14.2 14.9
  3' half 13.7 14.6 13.4 15.2 14.3 15.1
  Differencee 0 0.3 -0.1 0 0.1 0.2
YHB1 (cleavage at the first and last thirds)c 5' third 14 13.6 11.9 13.7 13.4 14.8
  3' third 13.5 13.2 11.4 14.1 12.6 14.5
  Differencee -0.5 -0.4 -0.5 0.4 -0.8 -0.3
YGR026W (cleavage at the middle of the ORF)d 5' half 12.4 14.7 13.8 13.1 12.9 15.4
  3' half 12.5 14.9 13.9 13.5 12.6 15.4
  Differencee 0.1 0.2 0.1 0.4 -0.3 0
  1. Numbers indicate the weighted-average fraction for each fragment. They were obtained by summing the products of the relative signal in a fraction times the fraction number for all fractions with a signal above background.
  2. a – This experimental repeat is presented in Figures 4 and 5.
  3. b – Cleavage scheme as depicted in Figure 4A.
  4. c – Cleavage scheme as depicted in Figure 5. Note that in the second and third repeats, the cleavage was done simultaneously with both ODNs added to the same sample. This enabled performing the entire procedure with a single sample (including separation on a single sucrose gradient and using northern analysis), therefore lowering the experimental error.
  5. d – Cleavage scheme as depicted in Figure 4B.
  6. e – Difference between the weighted-average fraction for the 3' fragment and the 5' fragment. This value represents the difference in sedimentation between the two fragments.