Design and evaluation of Actichip, a thematic microarray for the study of the actin cytoskeleton

BMC Genomics

Table 1 Functional groups of genes represented on the Actichip microarray

Gene function category		Number of genes represented on Actichip	Number of probes
Actin cytoskeleton	Actins	7	8
	Actin nucleators	9	14
	Actin bundling (cross-linking, severing)	52	56
	Actin sequestrating	7	7
	Barbed end actin capping	6	6
	Pointed end capping	7	7
	Filamentous actin stabilisation	5	5
	Actin depolymerisation	11	11
	Actin-dependent motors	42	44
	Actin-related, actin-like proteins	16	16
	Actin-binding proteins	14	14
	Cytoskeletal proteins	21	21
	Plasma membrane cortical cytoskeleton linkers/scaffold, signalling	52	60
Actin-based processes	Tissue-specific	4	5
	Cell adhesion	40	49
	Cancer	12	12
	Signalling	10	10
	DNA, transcription and translation factors	12	14
Total		327	359

A set of 359 long oligonucleotides (60-mer) were designed to represent 249 genes encoding proteins implicated in the actin cytoskeleton machinery or 78 genes involved in actin-based processes. Most of the genes (301) are represented by a unique probe, 22 genes by two probes, 3 genes by 3 probes and one gene by 5 probes.

ISSN: 1471-2164