Multiple sequence alignment of class II glycerate kinases (MOFRL family). The determined secondary structure of the Thermotoga maritima enzyme is shown above the sequences and the catalytic sites are marked (+). Amino acids identical to the first sequence are replaced by a dot; this allows the simple visualization of conserved and divergent regions. The determined consensus motif (PRATT, (D-X(0,2)-G-X(0,1)-D-[GP]-X(4)-[APS]-[ACDGST] (residues 322–332 of the T. tenax glycerate kinase)) is underlined and the MOFRL domain (T. maritima residue 304–410) is indicated by a bold ">"sign. Abbreviations: Avi, Agrobacterium vitis; Dme, Drosophila melanogaster; Hma, Haloarcula marismortui; Hsa, Homo sapiens; Mex, Methylobacterium extorquens; Pfu, Pyrococcus furiosus; Pto, Picrophilus torridus; Sso, Sulfolobus solfataricus; Tac, Thermoplasma acidophilum; Tma, Thermotoga maritima; Tte, Thermoproteus tenax.