|  | Archaea | Bacteria | ||
---|---|---|---|---|---|
 |  | Crenarchaea | Euryarchaea |  | |
 |  | T. tenax 1 | P. torridus 2 | T. acidophilum 3 | H. methylovorum 4 |
Growth optimum (°C) | 90 | 60 | 59 | 28 | |
Molecular mass | Â | Â | Â | Â | |
   Subunit (kDa) | 44 | 50 | 45 | 52 | |
   Native (kDa) | 48,3 | 95 | 49,3 | 41 | |
   Calculated (kDa) | 42,4 | 46,6 | 45,8 | (46.5)5 | |
   Oligomeric structure | Monomer | Dimer | Monomer | Monomer | |
Kinetic Parameters | Â | Â | Â | Â | |
D-Glycerate | Km (mM) | (0.02)6 | 0,34 | (0.56)7 | 0,13 |
 | Vmax (U mg-1) | (5.05)6 | 435 | (118)7 | 181 |
 | kcat (min-1) | 214 | 20271 | 5404 | 8417 |
 | kcat/Km (min-1mM-1) | 8913 | 59621 | 9651 | 64742 |
ATP | Km (mM) | (0.03)6 | 0,51 | (0.23)7 | 0,13 |
 | Vmax (U mg-1) | (4.41)6 | 432 | (118)7 | 181 |
 | kcat (min-1) | 187 | 20131 | 5404 | 8417 |
 | kcat/Km (min-1mM-1) | 7187 | 39473 | 16889 | 64742 |
Reaction product | 2-PG | 2-PG | 2-PG | 2-PG | |
Temperature optimum (°C) | 90 | 60 | 70 | 50 | |
Substrate specificity (%) | Â | Â | Â | Â | |
L-Glycerate | 30 | 34 | nd | 13 | |
Phosphate donor specificity (%) | Â | Â | Â | Â | |
   ATP | 100 | 100 | 100 | 100 | |
   GTP | 20 | 37 | 0–4 | 59 | |
   CTP | 16 | 24 | 0–4 | 59 | |
   UTP | 10 | 39 | 0–4 | 64 | |
   TTP | 16 | nd | nd | nd | |
Metal ion specificity (%) | Â | Â | Â | Â | |
   Mg2+ | 100 | 100 | 100 | 100 | |
   Co2+ | 56 | 11 | 8 | 75 | |
   Mn2+ | 59 | 11 | 10 | 72 | |
   Ni2+ | 30 | 25 | 0 | 29 | |
   Ca2+ | 15 | nd | 0 | 0 | |
Inhibition by ADP | + | nd | nd | + |