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Table 1 Comparison of kinetic and biochemical properties from characterized class II glycerate kinases (MOFRL family)

From: Glycerate kinase of the hyperthermophilic archaeon Thermoproteus tenax: new insights into the phylogenetic distribution and physiological role of members of the three different glycerate kinase classes

   Archaea Bacteria
   Crenarchaea Euryarchaea  
   T. tenax 1 P. torridus 2 T. acidophilum 3 H. methylovorum 4
Growth optimum (°C) 90 60 59 28
Molecular mass     
   Subunit (kDa) 44 50 45 52
   Native (kDa) 48,3 95 49,3 41
   Calculated (kDa) 42,4 46,6 45,8 (46.5)5
   Oligomeric structure Monomer Dimer Monomer Monomer
Kinetic Parameters     
D-Glycerate Km (mM) (0.02)6 0,34 (0.56)7 0,13
  Vmax (U mg-1) (5.05)6 435 (118)7 181
  kcat (min-1) 214 20271 5404 8417
  kcat/Km (min-1mM-1) 8913 59621 9651 64742
ATP Km (mM) (0.03)6 0,51 (0.23)7 0,13
  Vmax (U mg-1) (4.41)6 432 (118)7 181
  kcat (min-1) 187 20131 5404 8417
  kcat/Km (min-1mM-1) 7187 39473 16889 64742
Reaction product 2-PG 2-PG 2-PG 2-PG
Temperature optimum (°C) 90 60 70 50
Substrate specificity (%)     
L-Glycerate 30 34 nd 13
Phosphate donor specificity (%)     
   ATP 100 100 100 100
   GTP 20 37 0–4 59
   CTP 16 24 0–4 59
   UTP 10 39 0–4 64
   TTP 16 nd nd nd
Metal ion specificity (%)     
   Mg2+ 100 100 100 100
   Co2+ 56 11 8 75
   Mn2+ 59 11 10 72
   Ni2+ 30 25 0 29
   Ca2+ 15 nd 0 0
Inhibition by ADP + nd nd +
  1. 1this study; 2 [32]; 3 [30] 4 [33]; 5since no information of the encoding gene is available, the mean value of subunit and native molecular mass was formed and used for the calculation of the kcat/Km value; 6since glycerate inhibition is observed for the T. tenax enzyme the estimated values for glycerate concentration up to 0.3 mM are given; 7determined for DL-Glycerate; nd, not determined; +, detected.