The ecfA ( rpoE ) gene duplication. (a) PCR analysis of the duplicated 40 kb ecfA region among Bcc genomes. Strains used are described in Table 2. PCR primers are presented in Table 3. The DNA size marker is shown. Orientation of the PCR primers is indicated in the b and c sections. B/C primers detect the Bcen-J2315 configuration (duplication event)-ecfA2 allele, and A/B primers detect the Burkholderia sp. 383 configuration (no inversion). (b) and (c) Promer plot comparison of Burkholderia sp. 383 chromosome 1 against either (b) Bcen-J2315 chromosome 1 or (c) the partially inverted Bcen-J2315 chromosome 1. inverted region #1 an inverted region #2 of Bcen-J2315. ISBcen8 position is indicated. Promer analyses were performed according to .