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Table 4 Primary, Intermediate, and Secondary Metabolic Pathways in Citrus

From: Analysis of 13000 unique Citrus clusters associated with fruit quality, production and salinity tolerance

Pathway Enzymatic Activity EC reaction Unigenesa ESTsb
Glycolysis Hexokinase 2.7.1.1 4 (2)c 6 (4)d
  Glucose-6-phosphoisomerase 5.3.1.9 6 (1) 16 (1)
  6-Phosphofructokinase 2.7.1.11 10 35
  Fructose 1,6-biphosphate aldolase 4.1.2.13 12 (7) 71 (9)
  Triose phosphate isomerase 5.3.1.1 8 (1) 22 (1)
  Glyceraldehyde 3-P dehydrogenase 1.2.1.12 8 (2) 52 (8)
  Phosphoglycerate kinase 2.7.2.3 6 (1) 15 (3)
  Phosphoglycerate mutase 5.4.2.1 1 5
  Enolase 4.2.1.11 7 (4) 28 (5)
  Pyruvate kinase 2.7.1.40 10 (1) 69 (9)
Tricarboxylic acid cycle (Krebs cycle) Citrate synthase 4.1.3.7 2 13
  Aconitase 4.2.1.3 6 (1) 31 (1)
  Isocitrate dehydrogenase 1.1.1.42 3 59
  a-Ketoglutarate dehydrogenase complex 1.2.4.2 No hits No hits
  Succinyl-CoA synthetase 6.2.1.5 2 14
  Succinate dehydrogenase 1.3.5.1 2 20
  Fumarase 4.2.1.2 3 (2) 30 (5)
  Malate dehydrogenase 1.1.99.16 4 24
Oxidative/nonoxidative pentose phosphate pathway Glucose 6-P-1-dehydrogenase 1.1.1.49 4 4
  6-Phosphogluconolactonase 3.1.1.31 2 10
  6-Phosphogluconate dehydrogenase 1.1.1.44 15 47
  Ribose-5-P isomerase 5.3.1.6 3 12
  Ribose-5-P 3-epimerase 5.1.3.1 No hits No hits
  Transketolase 2.2.1.1 3 (2) 22 (11)
  Transaldolase 2.2.1.2 4 (2) 20 (17)
Aromatic amino acid biosynthesis 3-Deoxy-D-arabino-heptulosonate 7-P synthase 4.1.2.15 No hits No hits
  3-Dehydroquinate synthase 4.2.3.4 2 2
  3-Dehydroquinate dehydratase 4.2.1.10 2 2
  Shikimate 5 dehydrogenase 1.1.1.25 1 14
  Shikimate kinase 2.7.1.71 7 10
  5-Enolpyruvoylshikimate 3-P synthase 2.5.1.19 No hits No hits
  Chorismate synthase 4.2.3.5 2 2
  Chorismate mutase 5.4.99.5 1 1
  Prephenate dehydratase 4.2.1.51 3 5
  Prephenate dehydrogenase 1.3.1.12 2 (1) 18 (2)
  Aromatic amino acid transaminase 2.6.1.57 6 18
  Anthranilate synthase 4.1.3.27 6 (1) 46 (29)
  Anthranilate phosphoribosyl transferase 2.4.2.18 4 6
  Phosphoribosylanthranilate synthase 5.3.1.24 No hits No hits
  Indol-3-glycerol phosphate synthase 4.1.1.48 4 (2) 6 (2)
  Trp synthase 4.2.1.20 8 (1) 30 (1)
  Phe ammonia-lyase 4.3.1.5 5 13
  Cinnamate 4-hydroxilase 1.14.13.11 1 3
  4-Coumarate coenzyme A ligase 6.2.1.12 10 (1) 40 (1)
Flavonol biosynthesis Naringenin chalcone synthase 2.3.1.74 6 48
  Chalcone isomerase 5.5.1.6 3 17
  Flavanone 3-hydroxylase 1.14.11.9 2 (1) 12 (1)
  Flavonol 3-hydroxylase 1.14.13.21 6 7
  Flavonol synthase No E.C. 2 19
  Flavonol 3-O-glucosyltransferase No E.C. No hits No hits
Flavonoid biosynthetic pathway: anthocyanin biosynthesis Dihydroflavonol 4-reductase 1.1.1.219 No hits No hits
  Leucoanthocyanidin dioxygenase NoE.C. 2 33
  UDP-flavonol 3-O-glucosyltransferase 2.4.1.- No hits No hits
  Anthocyanin 5-O-glucosyltransferase NoE.C. 2 9
  Anthocyanin 5-aromatic acyltransferase 2.3.4.153 6 36
  Anthocyanin permease NoE.C. No hits No hits
Proanthocyanidin biosynthesis Anthocyanidin reductase 1.3.1.77 No hits No hits
  Leucoanthocyanindin reductase No E.C. 2 (1) 4 (2)
  1. aNumber of citrus unigenes annotated with the emzymatic activiy
  2. bTotal number of ESTs corresponding with the unigenes
  3. The number between brackets indicates the number of unigenes (c) or ESTs (d) that are first reported in this work