Giant panda BAC library construction and assembly of a 650-kb contig spanning major histocompatibility complex class II region

Table 1 Library screening results based on PCR amplification of 16 pairs of primers*

Locus	Type	Primer sequences (5'→3')	PCR product size (bp)	Number of positive clones	Source
Notch4	MHC class III	CGCTGTGCCTGCCTCAAT CATCACCTATGCCCTGAC	217	8	GenBank: AY973811
DRA	MHC class II	AGACAAGTTCTCGCCACC CTCCACCTTGCAGTCATAGA	172	2	GenBank: AY973816
IGHC	Genomic sequence	CAGCCCTCTTCCTCCTCACTC CGCCCAGCATCTGTTTCCA	244	5	GenBank: AY818395
MC1R	Genomic sequence	GCGGGCCATCTCCACTAT TTGAGGGCAGAGGACCAT	322	6	GenBank: AY884206
DRB	MHC class II	TCGCGTCCCCACAGCACATT GCCGCTGCACACTGAAACTCTCAC	281	12	[8]
DQA	MHC class II	GCTGACCATGTTGCTTACTAT AAGAGGCAGAGCATTGGACA	275	16	[9]
MHC13	MHC class I	GCTCCCACTCSMTGAGGTATT CTGCCTCGCTCTGGTTGTAG	293	6	GenBank: DQ367726
HSP70	MHC class III	TGCTGATCCAGGTGTACGAG CGTTGGTGATGGTGATCTTG	204	8	GenBank: M69100
G001	Micro-satellite	ACGGGAAGCCTGCTTCTACACTC AGACACCCAACCGACTAAACCAC	164–172	6	[22]
G905	Micro-satellite	CTGGCTTCAACTGCCTTTGAGAG GCACCTGGATTTGTGATGCTATC	169–177	5	[22]
Ameμ11	Micro-satellite	TATGCCACCTGCCCAGAC GATGGAAAGAGTAGAGCCAAGG	248–256	4	[23]
Ameμ15	Micro-satellite	AAGCAGTTGTTTTTGCTTAGTG TGTCAAAGTATTTGCCTCACA	142–150	3	[23]
Ameμ19	Micro-satellite	CAGGCAGCACAGCTATACCA CCACCTGATACCTATGCACAT	174–182	6	[23]
Ameμ22	Micro-satellite	AGGAAACATGTTGCCTTTTCA AGAGGGCAAATAGGAGGGAA	147–149	4	[23]
Ameμ25	Micro-satellite	CATAATTCCCTGGCAATGCT TGCCCGCATTGAAAAATG	239–257	3	[23]
Ameμ26	Micro-satellite	TTTTCAGGCCTCCGAAAAC ATTCCCAATAAAGCAAATCAGA	134–140	5	[23]

*To avoid false-positive results, individual positive clones were verified by the second round PCR. All the PCR products of positive clones were sequenced three times.

ISSN: 1471-2164