Disruption of the gene encoding Pol-γ. (A) The phylogenic tree of Pol-γ. (B) Tetrad dissection analysis of spores generated from pog1+/pog1Δ diploid cells after incubation at 30°C for ~5 days. The asterisk indicates that an uppermost wild type spore in the 9th tetrad was implanted into the agar and thus grows slower than other wild type spores. (C) The magnified view of a tetrad in (B). Genotypes of individual spores are indicated. (D) pog1Δ spores form petite colonies after a lengthy incubation term (~15 days) at 30°C.