Figure 4

Sequence alignment of eristalin with other dipteran defensins. (A) Eristalin was aligned with other dipteran defensins. For clarity only sequences of mature defensins were used for alignment. Red color indicates 90% and blue color 50% consensus. (B) A Bayesian protein tree was generated and we found that eristalin, defensin_1 and defensin_2 of Stomoxys calcitrans, and defensin_1 of Sarcophaga peregrina show some higher deviation in sequence when compared to other dipteran defensins. Accession numbers of defensins are (from top to bottom): AM706420, P10891, P18313, Q86BU1, P31530, P83404, P91793, P81602, P81603, Q17027, Q38L94, Q38LE1, Q4ZJJ8, P36192, Q86BW0, O16137, O16136. The scale bar represents the substitutions per site according to the model of amino acid evolution applied.