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Table 7 Primers sequences used for the transcripts amplifications

From: Identification of proteins involved in the functioning of Riftia pachyptila symbiosis by Subtractive Suppression Hybridization

Amplification of transcripts by classical PCR

 

Primers

Sequence

ExosqF

5' TGC AGG CGA TGC GAG TGC 3'

ExosqR

5' GCT ACA ACA GCG GTT AGG 3'

GalaxinF

5' ATT TCG TTT GCA ACA GCC 3'

GalaxinR

5' CTT CCT CTG CAG CAC TGG 3'

ChPrF

5' AAT TCT GAG ACC GGT GAC C 3'

ChPrR

5' TCC AAG ACC GTG TTG AGC 3'

RpCAtrF

5' TAC AAA GAT CCA ATC CAG C 3'

RpCAtrR

5' ACG AGG ACG ACA CCT GG 3'

RpCAbrF

5' TAC AAG GAT GCC ATT AGC 3'

RpCAbrR

5' AGA GCA GCA GAC CTT ACG 3'

IntraGlobF

5' GGA AAG GAC GTC GAC AGC 3'

IntraGlobR

5' TGC TGC TTG GTT AGT CCC 3'

MVPF

5' GAG AAC AGA ATG ACA TGG 3'

MVPR

5' TTT CAC CTG CAT CTC GGG 3'

ccoxIF

5' ACA GGT TTA GTA GCC ACT 3'

ccoxIR

5' GTG TTG ATA TAG GAC TGG 3'

Amplification of transcripts by quantitative PCR

 

Primers*

Sequence

RpCAbrFq

5' TGG TTT CAC CCC GTC GAA 3'

RpCAbrRq

5' GGT CTG GTC TTT TCT CGC CAT A 3'

RpCAtrFq

5' GCC AGG TGT CGT CCT CGT T 3'

RpCAtrRq

5' TCA CAA ATG TCC AGT GCC AGT T 3'

16SFq

5' CGT AAG ACT ATA GCT GGT TTT CCA AA 3'

16SRq

5' TTA TCA AAG ATT TTT TCT TGG TTC ACT AAT 3'

MVPFq

5' GAT TGA GAC AAC CAA GTT CAG GAA 3'

MVPRq

5' CTG GCG ATT GCC TGA ATT G 3'

CathepFq

5' TAC ATG GCC CGT AAT AAG GAC A 3'

CathepRq

5' GCT GGC TTG TGA TGC AAC AC 3'

ccoxIFq

5' CTA ATG GGA GGC TTC GGA AAC 3'

ccoxIRq

5' AGG TGC CCC TAG CAT TAA AGG 3'

ATPF1Fq

5' TGC AGG ACA TCA TTG CCA TC 3'

ATPF1Rq

5' TGT CCT CCT GGG ACA ACT CG 3'

ChPrFq

5' GTC GTC GGA ATG GCG AGT TA 3'

ChPrRq

5' AGC GTT GCT GGC TGT TTT G 3'

MHFq

5' AGA GGC ACA CCA ACA ACC GT 3'

MHRq

5' CCC GAT TGG TTC ATC ACA GC 3'

TCRFq

5' AAT CCG ACG TGG CGA TCA T 3'

TCRFq

5' GGT CAT TGT TGT TGC CTG GG 3'

TRbwC27Fq

5' CGA CGG TGG TAC CCC GTA TA 3'

TRbwC27Rq

5' CCG CAA CCT TTG AAC CTC AG 3'

  1. *Primers designed by Primer Express software (ABI PRISMâ„¢)