Table 5 Details on SNP markers scored by pyrosequencing method
Markers | Forward primer 5'-3' | Reverse primer Biotine 5'-3' | Product size (bp) | Sequencing primer 5'-3' | Alleles |
|---|---|---|---|---|---|
Chromosome X | Â | Â | Â | Â | Â |
BU21 | TGATGGTGGATAAGCAGGTGTA | GAAGTTGCGCTTTACCGTGTC | 77 | CCGCAATCTGGCTAAT | A/T |
G17 | GATACGCTCGAGATGAAGTACCTT | CTGTACATCCTCGTTGATCTTGC | 102 | ATGAAGTACCTTGAGCG | A/G |
4P9K1 | GGCTAGCGGAACGAAATTGATAC | TCGTTCGCCACCATATCCAT | 108 | GAAAAGATAAGCTACGAAAC | A/G |
Chromosome 2 | Â | Â | Â | Â | Â |
BU76 | GGCACCGGTCAAGGATGTA | GCCAGGAGGTTACAAACCACAG | 111 | AGGATGTATCCAGCGTTA | A/G |
7P6P4 | GACTAAGCGATTCAATTGGAGGAT | CAACGGTAAGCATGGATACTCTCT | 112 | ATTCAATTGGAGGATATTT | A/G |
3P6P3 | GAGAATGACGGCCAGGTGA | GGGTACTTGCGAAGTGTTTCTGAA | 142 | CAATGTGATAAACGGTATG | C/T |
AChE | TTGGTTCGTGCGGACATC | CGGTTCTGATTCTTTAAGCACAGC | 175 | AGTATTTTTGGGGACG | C/T |
3BU82 | GCACGGGAACTGGAAATGAT | GCATCGGAGCGTTTCCTATA | 84 | ACTGGAAATGATCGTTTA | A/G |
6BU40 | TTCCAGGCCAATGTCAGCA | AACGAAAACAAGGGGGAGAATG | 78 | CCAGGCCAATGTCAG | G/T |
CYP6Z1 | TGTCTGCACCAGCAGCATAGAAC | CGGGATTTCATCCAAATGCTA | 121 | CTTCCGCATTGTTTC | Â |
BU29 | GCACGGAGAAGGTGATCAACTA | GAGGTTGGTCGCAATTATTCG | 188 | CCTCCAGAAGAAGTACGAT | A/G |
BU901 | GGTGAGTTTTTTGGTTGCTTAATG | TCAATTTCTAAAATGGCATCAACA | 97 | GAGTTTTTTGGTTGCTT | A/G |
1BU62 | CGGTAAGTATAATATACGCCCAGT | GTTTGAGGAACGCATGAGC | 75 | TATAATATACGCCCAGTACA | C/T |
Chromosome 3 | Â | Â | Â | Â | Â |
BU66 | AAGTTCCAGGATGAAGGTGTGTT | GCCTTCGGGATGCCATTAT | 129 | ATGCGAGCTAGCCAA | A/G |
BU92 | ACGACTTCACCGGAGTCTCT | CGCTTCTTCCAGGGACTTATT | 87 | ACGACTTCACCGGAGTCT | C/T |
5P9J12 | CCAACAAATCAGTTCATCAGC | CTTTGGCGCGAAGGTAAA | 97 | TTGTAAAAGTGCTTAAAATG | A/C |
2P9J14 | TACCGTCTGTTTCCTTGGATAATG | ATTTCGGCGAAGAACAAATTTAG | 80 | GCTTGAAGTGGACCTG | G/T |
BU021 | TTTAGGGGTTCCAATTGACGC | ACCCATACCGGAACCAGTACCAC | 56 | ATTGACGCACTCGCT | G/T |
1P9J10 | TGCGTCTTTCGGTACAGGAT | AACGTATGTTCTTACCCACAACCA | 141 | AATCTTTACGGTTGTATCC | A/G |