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Table 4 Strains and plasmids used in this study.

From: The small FNR regulon of Neisseria gonorrhoeae: comparison with the larger Escherichia coli FNR regulon and interaction with the NarQ-NarP regulon

Strain Description Source
N. gonorrhoeae strains
F62 Parental strain Laboratory stocks
RUG7001 F62 proAB paniA::lacZ [43]
RUG7022 F62 proAB paniA::lacZ fnr [6]
JCGC201 F62 proAB pccp::lacZ [5]
JCGC202 F62 proAB pccp::lacZ fnr [5]
JCGC502 F62 fnr- 3xFLAG KanR This work
E. coli strains
RV Parental strain Laboratory stocks
JCB386 Δ (nirB-cycG) pnirB::lacZ [44]
JCB3861 JCB386 narXL This work
JCB3862 JCB386 narXL narQ This work
JCB3863 JCB386 narXL narQ narP This work
JCB391 RV narXL narQ pcnB This work
JCB12 RV narX narQ frdA::lacZ [10]
Plasmids
pGCFNR3 Gonococcal fnr, under the regulation of the E. coli fnr promoter, cloned into pBR322. [5]
pGCFNRi pGCFNR3 with Kpn I and Xho I restriction sites engineered downstream of the fnr gene This work
pGCFNR-FLAG pGCFNR3 with a 3xFLAG tag and Kanamycin resistance cassette inserted downstream of the fnr gene This work
pSUB11 Epitope tagging plasmid carrying 3xFLAG tag and kanamycin resistance cassette. [36]
pGCNarQP Gonococcal narQP genes under the control of the E. coli fnr promoter cloned into pBR322. This work
pBADgcQ Gonococcal narQ gene under the control of the araBAD promoter cloned into pBAD myc-His A. This work
pBADecQ E. coli narQ gene under the control of the araBAD promoter cloned into pBAD myc-His A This work
pRNW18 pBADEcQ containing R54K substitution in narQ This work
pRNW19 pBADEcQ containing N48E I49E substitution in narQ This work
pRNW20 pBADEcQ containing N48E I49E R54K substitution in narQ This work
pRNW29 pBADEcQ containing D43A E45S A46V N48E I49E substitution in narQ This work
pRNW30 pBADEcQ containing D43A E45S A46V substitution in narQ This work
pRNW31 pBADEcQ containing D43A E45S A46V R54K substitution in narQ This work
pRNW32 pBADEcQ containing D43A E45S A46V N48E I49E R54K substitution in narQ This work
pRNW33 pBADEcQ containing D43A E45S A46V S52N S57A substitution in narQ This work
pRNW34 pBADEcQ containing D43A E45S A46V N48E I49E S52N R54K S57A substitution in narQ This work
p7150 E. coli nirB promoter cloned into the lacZ reporter vector pRW50. [19]
pRNW15 E. coli napF promoter cloned into the lacZ reporter vector pRW50. This work
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