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Table 1 Influence of Stress on the Viability and Global Transcription Profile

From: Cadmium triggers an integrated reprogramming of the metabolism of Synechocystis PCC6803, under the control of the Slr1738 regulator

Treatment Cadmium Hydrogen peroxide Fe depletion Fe excess Zn excess
[C] 50 μM 3 mM 2-0 1-0 16 mM 776 μM
Time (min) 15 30 60 75 90 180 300 300' 360 960 15 30 180 300 420 2,800 2,800 240 360 30 240
% survival 100 98 90 88 85 70 57 59 43 <10 100 98 64 45 19 55 43 80 15 99 70
Induced genes 22 88 46 52 293 299 310 451 283 51 447 408 170 68 75 106 154 42 34 38 245
Repressed gene 8 17 10 13 250 315 328 439 310 26 490 478 92 12 55 104 109 98 100 22 221
  1. Wild type cells were challenged for the indicated durations prior to survival and transcriptome analyses (Methods). In the case of the Fe starvation stress, the switch in Fe concentration is indicated as 1-0 (standing for 1 μM then 0 μM) or 2-0 (standing for 2 μM then 0 μM). The time point 300 min of the kinetic of Cd responses was repeated twice (independent biological repeats, columns 300 and 300') with the two different versions of the Cyanochip DNA microarrays, leading to very reproducible results (see Additional files 2 and 4).
  2. Genes were considered differentially regulated whenever their level of expression was changed at least 1.9 fold.