Treatment | Cadmium | Hydrogen peroxide | Fe depletion | Fe excess | Zn excess |
---|
[C]
|
50 μM
|
3 mM
|
2-0
|
1-0
|
16 mM
|
776 μM
|
---|
Time (min) | 15 | 30 | 60 | 75 | 90 | 180 | 300 | 300' | 360 | 960 | 15 | 30 | 180 | 300 | 420 | 2,800 | 2,800 | 240 | 360 | 30 | 240 |
---|
% survival | 100 | 98 | 90 | 88 | 85 | 70 | 57 | 59 | 43 | <10 | 100 | 98 | 64 | 45 | 19 | 55 | 43 | 80 | 15 | 99 | 70 |
Induced genes | 22 | 88 | 46 | 52 | 293 | 299 | 310 | 451 | 283 | 51 | 447 | 408 | 170 | 68 | 75 | 106 | 154 | 42 | 34 | 38 | 245 |
Repressed gene | 8 | 17 | 10 | 13 | 250 | 315 | 328 | 439 | 310 | 26 | 490 | 478 | 92 | 12 | 55 | 104 | 109 | 98 | 100 | 22 | 221 |
- Wild type cells were challenged for the indicated durations prior to survival and transcriptome analyses (Methods). In the case of the Fe starvation stress, the switch in Fe concentration is indicated as 1-0 (standing for 1 μM then 0 μM) or 2-0 (standing for 2 μM then 0 μM). The time point 300 min of the kinetic of Cd responses was repeated twice (independent biological repeats, columns 300 and 300') with the two different versions of the Cyanochip DNA microarrays, leading to very reproducible results (see Additional files 2 and 4).
- Genes were considered differentially regulated whenever their level of expression was changed at least 1.9 fold.