Cadmium triggers an integrated reprogramming of the metabolism of Synechocystis PCC6803, under the control of the Slr1738 regulator

Table 1 Influence of Stress on the Viability and Global Transcription Profile

Treatment	Cadmium										Hydrogen peroxide					Fe depletion		Fe excess		Zn excess
[C]	50 μM										3 mM					2-0	1-0	16 mM		776 μM
Time (min)	15	30	60	75	90	180	300	300'	360	960	15	30	180	300	420	2,800	2,800	240	360	30	240
% survival	100	98	90	88	85	70	57	59	43	<10	100	98	64	45	19	55	43	80	15	99	70
Induced genes	22	88	46	52	293	299	310	451	283	51	447	408	170	68	75	106	154	42	34	38	245
Repressed gene	8	17	10	13	250	315	328	439	310	26	490	478	92	12	55	104	109	98	100	22	221

Wild type cells were challenged for the indicated durations prior to survival and transcriptome analyses (Methods). In the case of the Fe starvation stress, the switch in Fe concentration is indicated as 1-0 (standing for 1 μM then 0 μM) or 2-0 (standing for 2 μM then 0 μM). The time point 300 min of the kinetic of Cd responses was repeated twice (independent biological repeats, columns 300 and 300') with the two different versions of the Cyanochip DNA microarrays, leading to very reproducible results (see Additional files 2 and 4).
Genes were considered differentially regulated whenever their level of expression was changed at least 1.9 fold.

ISSN: 1471-2164