Figure 3
Phylogenetic analysis of plasma membrane Ca2+-ATPase (PMCA) and Na+/Ca2+ exchanger (NCX) amino acid sequences. Consensus trees were generated using a Neighbor-joining (NJ) method with 1000-time bootstraps. The GenBank accession numbers of the sequences used are as follows: human PMCA 1 (hPMCA1), P20020; human PMCA 2 (hPMCA2), Q01814; human PMCA 3 (hPMCA3), Q16720; human PMCA 4 (hPMCA4), P23634; Norway rat PMCA 1 (ratPMCA1), P11505; Norway rat PMCA 2 (ratPMCA2), P11506; Norway rat PMCA 3 (ratPMCA3), Q64568; Norway rat PMCA 4 (ratPMCA4), Q64542; mouse PMCA 2 (mPMCA2), NP_033853; mouse PMCA 3 (mPMCA3), NP_796210; mouse PMCA 4.b (mPMCA4.b), AY560895; dog PMCA 1 (dogPMCA1), NP_777121; pig PMCA 1 (pigPMCA1), P23220; bullfrog PMCA 1bx (bullfrogPMCA1bx), AAK11272; bullfrog PMCA 2av (bullfrogPMCA2av), AAK11273; Caenorhabditis elegans PMCA (CelegansPMCA), CAA09303;Oreochromis mossambicus PMCA 2 (tilapiaPMCA2), AAK15034; Tetraodon nigroviridis unnamed protein product (tetradonPMCA1a), CAF95990; T. nigroviridis unnamed protein product (tetradonPMCA1b), CAG08760; T. nigroviridis unnamed protein product (tetradonPMCA4), CAF90203; human NCX 1 (hNCX1), P32418; human NCX 2 (hNCX2), Q9UPR5; human NCX 3 (hNCX3), NP_150287; mouse NCX 1 (mouseNCX1), P70414; mouse NCX 2 (mouseNCX2), NP_683748; mouse NCX 3 (mouseNCX3), NP_536688; Norway rat NCX 1 (ratNCX1), Q01728; Norway rat NCX 2 (ratNCX2), NP_511174; Norway rat NCX 3 (ratNCX3), NP_511175; T. nigroviridis unnamed protein product (tetraodonNCX1), CAG00274; T. nigroviridis unnamed protein product (tetraodonNCX2a), CAF95011; T. nigroviridis unnamed protein product (tetraodonNCX2b), CAG06357; T. nigroviridis unnamed protein product (tetraodonNCX3), CAG13245; T. nigroviridis unnamed protein product (tetraodonNCX4a), CAG05743; T. nigroviridis unnamed protein product (tetraodonNCX4b), CAG01582; Ore. mossambicus NCX1a (tilapiaNCX1a), AAP37041; Oncorhynchus mykiss NCX 1a (troutNCX1a) AAF06363; Rhodopirellula baltica NCX (PirellulaNCX), NP_868143; C. elegans NCX (CelegansNCX), CAA04574; Loligo opalescens NCX (squidNCX), AAB52920; and Drosophila melanogaster NCX (flyNCX), NP_524423. The un-annotated proteins of Takifugu rubripes obtained from Ensembl browser with the Ensembl gene ID are as follows: Takifugu PMCA 3b (fuguPMCA3b), NEWSINFRUG00000123527; Takifugu PMCA 2 (fuguPMCA2), NEWSINFRUG00000164849; Takifugu PMCA 1a (fuguPMCA1a), NEWSINFRUG00000133763; Takifugu PMCA 4 (fuguPMCA4), NEWSINFRUG00000161238; Takifugu PMCA 1b (fuguPMCA1b), NEWSINFRUG00000135851; Takifugu NCX 4b (fuguNCX4b), NEWSINFRUG00000165032; Takifugu NCX 4a (fuguNCX4a), NEWSINFRUG00000131148; Takifugu NCX 1a (fuguNCX1a), NEWSINFRUG00000136894; Takifugu NCX 1b (fuguNCX1b), NEWSINFRUG00000145936; Takifugu NCX 3 (fuguNCX3), NEWSINFRUG00000135815; Takifugu NCX 2b (fuguNCX2b), NEWSINFRUG00000136441; and Takifugu NCX 2a (fuguNCX2a), NEWSINFRUG00000160221. An asterisk (*) indicates a hypothetical protein predicted from other studies.