Effect of inhibition of various branches of MAPKs on the cytotoxicity and TG accumulation in response to palmitate. Cells were treated for 48 hours with palmitate in the presence of 75 uM SP-600125 (to inhibit JNK) or 50 uM U-0126 (to inhibit ERK). Media were changed daily. (a) Effect on cytotoxicity. The LDH released into the medium after 24 and 48 h of treatments was measured and normalized to the total LDH. (b) Effect on intracellular TG. Intracellular levels of TG were measured after desired time of exposure. Data presented as mean +/- s.d. of 3 independent experiments. , significantly different than palmitate (p < 0.01).