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Figure 3 | BMC Genomics

Figure 3

From: A screen for nuclear transcripts identifies two linked noncoding RNAs associated with SC35 splicing domains

Figure 3

Northern Analysis of NEAT1 and NEAT2/MALAT-1 in mouse and human tissues. Radiolabeled probes to NEAT1 and NEAT2/MALAT-1 show a ~3.5 kb transcript for the human and mouse NEAT1 loci (A,C) and a transcript of more than 6 kb for the human and mouse NEAT2 loci (B,D). The analyses were carried out using First Choice Northern Blots (Ambion), which contain 2 micrograms each of poly(A) RNA from various tissues. Equal loading of RNA is ensured by Ambion for each lot of blots with either GAPDH or beta-actin probes for human or mouse blots respectively. Difficulties in transferring large transcripts to Northern blots may explain the absence of a larger (>17 kb), NEAT1 transcript (data not shown and [29]).

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BMC Genomics

ISSN: 1471-2164

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