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Table 2 Success rates of ORF amplification

From: The full-ORF clone resource of the German cDNA Consortium

template type cDNA clone EST clone primary cDNA total
ORFs targeted # total # posa %b %c # total # posa %b %c # total # posa %b %c # total # posa %b %c
1. amplification 1712 1303 76 76 368 267 73 73 156 39 25 25 2236 1609 72 72
reamplification d 409 162 40 9 101 39 39 11 117 25 21 16 627 226 36 10
amplified with alternative template 103 80 78 5 28 22 79 6 61 15 25 10 192 117 61 5
amplified with alternative 1-step primers 32 26 81 2 16 13 81 4 21 6 29 4 69 45 65 2
Sum 1712e 1571   92 368e 341   94 156e 85   55 2236f 1997   89
  1. a Number of ORFs with expected product size obtained by PCR.
  2. b Sucess rate of the respective reaction step.
  3. c Contribution of each step to the final PCR success rate (rounded values).
  4. d Reamplification with slight modifications of the PCR protocol depending on result of first amplification.
  5. e Sum of ORFs targeted per template type.
  6. f Total sum of ORFs targeted