The full-ORF clone resource of the German cDNA Consortium

BMC Genomics

Table 2 Success rates of ORF amplification

template type	cDNA clone				EST clone				primary cDNA				total
ORFs targeted	# total	# pos^a	%^b	%^c	# total	# pos^a	%^b	%^c	# total	# pos^a	%^b	%^c	# total	# pos^a	%^b	%^c
1. amplification	1712	1303	76	76	368	267	73	73	156	39	25	25	2236	1609	72	72
reamplification ^d	409	162	40	9	101	39	39	11	117	25	21	16	627	226	36	10
amplified with alternative template	103	80	78	5	28	22	79	6	61	15	25	10	192	117	61	5
amplified with alternative 1-step primers	32	26	81	2	16	13	81	4	21	6	29	4	69	45	65	2
Sum	1712^e	1571		92	368^e	341		94	156^e	85		55	2236^f	1997		89

^a Number of ORFs with expected product size obtained by PCR.
^b Sucess rate of the respective reaction step.
^c Contribution of each step to the final PCR success rate (rounded values).
^d Reamplification with slight modifications of the PCR protocol depending on result of first amplification.
^e Sum of ORFs targeted per template type.
^f Total sum of ORFs targeted

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