Sequence analysis of an Archaeal virus isolated from a hypersaline lake in Inner Mongolia, China

Pagaling, Eulyn; Haigh, Richard D; Grant, William D; Cowan, Don A; Jones, Brian E; Ma, Yanhe; Ventosa, Antonio; Heaphy, Shaun

doi:10.1186/1471-2164-8-410

BMC Genomics

Table 1 Predicted ORFs in virus BJ1

From: Sequence analysis of an Archaeal virus isolated from a hypersaline lake in Inner Mongolia, China

ORF	Start	Stop	aa	Mr	pI	RBS/distance
1	130	990	286	33	4.6	-
2	1146	1805	219	25	4.9	-
3-	1980	2093	37	3.7	8.5	GGAGGTG-5
4	2207	2425	72	8.1	7.0	-
5-	2541	3191	216	24	4.7	GAGG-10
6-	3178	3393	71	8.2	4.3	-
7 V	3547	3993	148	16	4.1	GAG-6
8	3993	4463	156	18	4.3	AGGAGGTGA-8
9	4456	4851	131	15	4.2	AGGAGGTGA-7
10	4844	5218	124	14	4.7	GGAGGT-6
11	5208	5357	49	5.2	3.8	GAGGTG-8
12	5350	5574	74	8.3	4.6	AGGAGGT-6
13	5571	5744	57	6.1	10.4	GGAGG-5
14	5741	5986	81	8.9	5.8	GGAGG-8
15-	5998	6417	139	15	4.3	GAGG-7
16	6637	7713	358	40	5.0	AGGTG-9
17-	7919	8560	213	24	4.3	AGGA-8
18	8689	8949	86	9.3	4.9	-
19	8950	9153	67	7.9	5.2	GGTG-10
20-	9159	9446	95	11	4.6	GGAG-4
21	9660	10022	120	14	4.6	GGA-7
22	10022	10153	43	4.6	4.0	GGTG-8
23	10153	10890	245	28	3.9	GGAGG-8
24	10880	11806	308	34	4.3	GGAGG-9
25 V	11803	11946	47	5.2	4.1	GGTGA-7
26	11946	12671	241	27	4.7	GGTGA-7
27 V	12668	12760	30	3.3	4.5	GGAGGTG-6
28	12757	13092	111	12.2	5.8	GAGGTGA-5
29	13092	13262	56	6.2	3.8	GGAGG-8
30	13255	14700	481	52	6.2	AGGAGG-6
31-	13270	14487	405	46	5.0	-
32	14701	14826	41	4.3	4.0	GGAGGTGA-9
33	14819	15307	162	18	4.6	GAGGTGA-7
34	15310	15531	73	83	11.6	AGGAGGTG-9
35	15489	17603	704	78	4.7	(GAAAA)
36	17606	18058	150	17	4.4	GGAGG-9
ORF	Start	Stop	aa	Mr	pI	RBS/distance
37	18055	18519	154	18	4.3	(GGGGG)
38 V	18512	18817	101	11	5.0	GAGGTG-8
39 V	18814	19074	86	9.9	6.1	GAGGTG-9
40 V	19071	19241	56	5.9	10.3	GGAGG-8
41 V	19129	19806	225	26	6.3	-
42	19803	19982	59	6.4	4.0	GAGGTG-6
tRNA	19973	20046	-	-	-	-
43	20365	21843	492	55	4.9	-
44	21840	21998	52	5.9	4.3	-
45	22001	22111	36	3.9	4.8	-
46	22108	22416	102	11	4.3	-
47	22416	22577	53	6.1	4.2	-
48	22574	23083	169	19	4.3	-
49	23080	24423	447	50	4.9	GAGG-8
50	24427	26382	651	73	4.5	-
51	26461	26586	41	4.4	4.4	GAG-9
52	26590	27933	447	47.	3.9	AGGAGG-9
53	27949	29031	360	40	4.2	GTGA-8
54	29040	29219	59	6.4	3.8	GAGGTGA-4
55	29222	29572	116	12	3.9	-
56	29576	30451	291	33	4.6	GGAGG-9
57	30444	30761	105	11	4.1	-
58	30758	31210	150	17	4.8	AGG-10
59	31207	31734	175	20	4.5	GGAGGT-5
60 V	31766	32680	304	32	3.8	GAGGTGA-7
61	32680	33177	165	18	4.0	AGGAGGTGA-8
62	33281	34408	375	38	4.1	-
63	34444	34731	95	10	4.8	-
64	34771	35439	222	24	4.0	-
65	35446	36633	395	42	4.1	TGA-7
66	36634	38226	530	52	3.7	AGGAGGTG-10
67	38229	40979	916	100	4.0	GGAGGTG-15
68	41059	41400	113	12	3.8	GGAG-6
69	41403	41843	146	16	4.6	AGGTG-9
70	41840	42151	103	11	3.9	GGTGA-4

Orfs are in the forward direction unless indicated by a -ve sign. v indicates a valine start. aa indicates the number of amino acids. Mr is the molecular mass × 10^-3, rounded to the nearest 100. pI is the isoelectric point rounded to one decimal place. rbs/distance is the ribosome binding site sequence and its distance from the start codon.

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