Figure 7

Fold change in time-course gene expression of F3- or LPS-induced THP-1 cells by Q-PCR. First, THP-1 cells were treated with F3 or LPS for different periods of time and were collected at different time points. Total RNA was isolated from cell lines using TRIzol reagent. First-strand cDNA synthesis were performed with 5 μ g of total RNA in a volume of 20 μ l with 1 μ l ThermoScript™ Reverse Transcriptase (Invitrogen) and 1 μ l oligo(dT). Extracted first-strand cDNAs were analyzed using BioRad iCycler iQ Real-Time Detection System with SYBR Green dye (Molecular Probes, Eugene, OR). Software designed for the BioRad iCycler will aid in analyzing collected data. mRNA expression of these genes were normalized to RNA content for each sample by using GADPH gene products as internal controls. Relative expression was calculated as the ratio of expression from each F3-treated THP-1 cells in comparison to untreated THP-1 cells (control). The error bar came from n > 3. The p values indicate the statistical significance of different time-course gene expression profiles between F3 and LPS treatment.