Table 8 Primers used for the real-time PCR analysis
From: Differential gene expression profile in pig adipose tissue treated with/without clenbuterol
Genes & clone number | Primer sequence (5' to 3') | PCR product size (bp) | Annealing Temperature |
|---|---|---|---|
GAPDH | For. ATGGTGAAGGTCGGAGTGA Rev. ATGGGTAGAATCATACTGGA | 154 bp | 57°C |
Beta 2-microglobulin (B2M) | For. TGG TCTTTCTACCTTCTGGCCC Rev. TGTGATGCCGGTTAGTGGTCTC | 166 bp | 60°C |
Apolipoprotein D (rpfat_18926) | For. AGATCCCAGTGAGCTTTGAG Rev. CGTAGTTCTCATAGTCGGTG | 233 bp | 58°C |
PRKAR1A (rpfat_17661) | For. GGCGACGAGGTGCTATCAG Rev. ATGGCATCAAAAATATCAC | 159 bp | 60°C |
COL1A1 (rpfat_8523) | For. TCAAGATGTGCCACTCCGACT Rev. GCCTGTCTCCATGTTGCAGAA | 104 bp | 60°C |
COL1A2 (rpfat_16033) | For. ATATGCACCTTGGACATCGGT Rev. CACGATGCTCTGATCAATCCT | 241 bp | 60°C |
COL3A1 (rpfat_19990) | For. CCTGCTGGAAAGAATGGTGAC Rev. ACGTTCACCGGTTTCACCTT | 132 bp | 60°C |
COL1A2 (rpfat_17393) | For. CCTGGCTCTAGAGGTGAACG Rev. AGCAGGACCAGGATTACCAG | 247 bp | 60°C |
COL3A1 (rpfat_18309) | For. TTTCTTTTATGGCTCCCCCTG Rev. GCGTGTTCGATATTCGAAGAC | 101 bp | 60°C |
SCD (rpfat_16685) | For. AAGGAACTAGAAGGCTGCTC Rev. TGTAGAGCAGCAGCCATCAC | 156 bp | 58°C |
PHPT1 (rpfat_15312) | For. GAAGACACAGTTGAGGACAC Rev. GGACATTGTTCGGAGGATAG | 110 bp | 60°C |
HSL (rpfat_11096) | For. TCCGAATGGAGTCTGCACTGT Rev. CTTCCACTCTGACCTCCAACG | 128 bp | 60°C |
PMP22 (rpfat_18575) | For. CATGAACATTTGCACCACTTG Rev. GTCAGCACCTAATGGTATGGA | 133 bp | 60°C |