Figure 3

Structure-based alignment of the Smoc-1 protein from different mammalian species. The sequences were aligned across the species for FS (A), TY1-1 (B), Smoc-1 (C), TY1-2 (D) and EC (E) domains. Mutational hotspots in buffalo Smoc-1 are shown red boldface. Most of the observed changes in buffalo Smoc-1 were shared either by cattle or human. The "→" indicates the potential N-glycosylation site. Pairs of numbers above the sequence correspond to cysteines indicating the predicted disulphide bonds based on the disulphide linkage of BM-40 and thyroglobulin. Both EF hand motifs are underlined and calcium coordinating residues are overshadowed grey. Conserved amino acids are indicated by the stars below and cysteines with a black background.