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Table 2 Clusters showing significant differences as determined by Stekel R value and Chi square analysis.

From: Construction and characterization of an expressed sequenced tag library for the mosquito vector Armigeres subalbatus

GBID Product asuhem diroinf imacbac brumal naïve7 naïve14 R Chi
EU204979 NADH dehydrogenase subunit 2 245 23 14 10 21 19 0 0
EU204980 NADH dehydrogenase subunit 6 172 18 8 1 10 11 0 0
EU204999 cytochrome c oxidase subunit I 245 67 24 11 59 54 0 0
EU205005 unknown 325 52 33 17 26 66 0 0
EU205016 cytosolic small ribosomal subunit S26 60 69 57 41 36 67 0.000001 0.000001
EU205017 NADH dehydrogenase 28 2 2 4 6 1 0.000002 0.000001
EU205057 ubiquitin 83 0 0 0 1 0 0 0
EU205058 cytochrome c oxidase subunit III 277 79 83 20 62 52 0 0
EU205059 cytochrome b 47 19 14 1 9 8 0 0
EU205061 mitochondrial adenosine triphosphatase subunit 6 202 60 36 1 51 45 0 0
EU205096 ubiquinol-cytochrome c reductase subunit 62 9 4 12 9 11 0 0
EU205097 cytochrome c 74 15 8 12 11 12 0 0
EU205100 unknown 243 59 123 79 54 72 0 0
EU205101 putative: conserved unknown 32 18 7 3 20 6 0.000001 0.000002
EU205152 ubiquinol-cytochrome c reductase 56 14 5 11 14 9 0 0
EU205153 AT DNA binding 0 7 15 13 6 8 0.000002 0.00033
EU205154 questionable: Bombyx mori prophenoloxidase activating factor 3 26 1 3 3 1 1 0.000001 0
EU205155 questionable: conserved unknown 1 6 7 21 8 10 0.000006 0.00008
EU205171 ubiquinol-cytochrome-c reductase 94 16 8 29 20 26 0 0
EU205182 cytosolic large ribosomal subunit L36 27 43 33 24 26 47 0 0
EU205185 NADH dehydrogenase 47 2 8 10 4 7 0 0
EU205197 gelsolin 129 20 38 33 13 57 0 0
EU205226 cytosolic large ribosomal subunit L40 21 5 0 0 5 1 0 0.000005
EU205248 cytochrome C oxidase subunit II 52 15 12 6 9 27 0.000001 0.000002
EU205296 Myosin alkali light chain 1 35 3 8 0 3 6 0 0
EU205304 questionable: receptor binding 0 0 0 2 6 0 0.000228 0.000002
EU205306 questionable: cytochrome c oxidase 63 13 6 6 13 3 0 0
EU205324 conserved unknown 17 0 4 0 0 4 0.000003 0.000054
EU205330 cytosolic small ribosomal subunit S9 39 73 43 68 32 54 0 0
EU205338 superoxide dismutase 20 2 1 0 1 1 0.000002 0.000001
EU205349 ribosomal protein L41 276 100 170 94 126 104 0 0
EU205374 nucleoside diphosphate kinase 2 14 1 11 0 5 0.000002 0.000002
EU205385 questionable: protein-tyrosine kinase 0 0 0 107 0 0 0 0
EU205393 hydrogen transporting two sector ATPase 53 11 11 8 17 9 0.000003 0.000002
EU205409 cecropin 17 1 1 0 0 5 0.000006 0.000023
EU205450 serine protease 1 6 28 9 3 19 0 0
EU205451 cytosolic small ribosomal subunit S3A 78 26 27 16 20 29 0.000068 0.000132
EU205465 defensin 136 14 27 6 5 9 0 0
EU205470 putative: heat shock 0 0 1 56 2 3 0 0
EU205505 lysozyme 116 17 32 10 11 35 0 0
EU205526 cytochrome c oxidase 130 15 11 10 16 5 0 0
EU205556 putative: conserved unknown 0 0 3 41 0 3 0 0
EU205557 putative: conserved unknown 0 0 0 9 0 1 0.000047 0.000003
EU205560 cecropin 32 35 17 3 14 11 0 0
EU205570 putative: conserved unknown 0 9 13 15 7 7 0.000003 0.000718
EU205592 conserved unknown 63 21 11 12 12 16 0.000007 0.000004
EU205632 putative: calcium ion binding protein 0 0 13 0 0 0 0 0
EU205658 serine protease 112 18 42 43 7 29 0 0
EU205659 unknown 35 0 3 0 2 0 0 0
EU205708 proton-transporting ATP synthase complex subunit 70 8 9 4 14 16 0 0
EU205709 putative: conserved unknown 25 0 3 2 2 2 0.000001 0
EU205750 hydrogen-exporting ATPase 33 2 5 2 1 9 0 0
EU205751 putative: odorant-binding 0 0 0 9 0 0 0.000015 0
EU205764 questionable: odorant-binding protein 56e 0 0 0 9 0 0 0.000015 0
EU205767 trypsin 0 0 0 25 0 0 0 0
EU205782 trypsin 10 221 98 70 107 70 0 0
EU205800 unknown 5 4 9 7 11 39 0 0
EU205806 questionable: odorant-binding 0 0 0 123 0 0 0 0
EU205807 questionable: odorant-binding 0 0 0 28 0 0 0 0
EU205828 trypsin 3 85 10 11 33 2 0 0
EU205832 questionable: apolipophorin 129 0 5 32 2 6 0 0
EU205858 vitellogenin C 0 0 0 18 0 0 0 0
EU205893 unknown 130 0 0 0 0 1 0 0
EU205915 hydrogen transporting two sector ATPase 41 6 5 2 2 4 0 0
EU205924 putative: serine protease 5 13 28 2 14 6 0 0
EU205925 putative: serine protease 1 15 10 4 12 5 0.000001 0.000002
EU205991 unknown 0 3 1 39 2 4 0 0
EU205996 ATPase synthase 16 4 1 0 0 0 0.000003 0.000012
EU206001 NADH dehydrogenase 36 6 3 8 7 1 0.000001 0.000001
EU206048 conserved unknown 45 8 11 10 4 6 0.000021 0.000005
EU206083 putative: serine protease 0 0 0 61 0 0 0 0
EU206123 putative: serine protease 0 5 11 28 7 8 0 0
EU206138 questionable: conserved unknown 0 0 0 32 0 0 0 0
EU206244 cytosolic large ribosomal subunit L34a 1 38 10 9 7 3 0 0
EU206248 zinc-metalloproteinase precursor 2 14 1 0 12 3 0 0
EU206376 putative: cathepsin 0 0 0 30 0 0 0 0
EU206422 infection responsive short peptide (gambicin) 17 23 40 47 17 24 0.000001 0.000002
EU206504 unknown 0 0 0 12 0 0 0 0
EU206507 ATP/ADP antiporter (transporter) 21 3 1 0 1 0 0 0
EU206546 beta-globin 0 0 0 34 0 0 0 0
EU206601 cathepsin 0 0 0 19 0 0 0 0
EU206635 trypsin 0 4 0 16 3 2 0 0
EU206733 putative: conserved unknown 0 0 0 9 0 0 0.000015 0
EU206824 putative: arrestin 2 0 13 6 8 9 9 0.000004 0.000307
EU206866 questionable: conserved unknown 0 0 0 32 0 0 0 0
EU206900 rhodopsin 0 2 13 11 5 11 0.000001 0.000066
EU206907 questionable: chitin binding 0 0 0 12 0 0 0 0
EU206986 unprocessed 18S ribosomal RNA 40 1 0 1 0 0 0 0
EU207626 unknown 90 0 0 1 0 2 0 0
EU207634 unknown 107 56 65 38 22 89 0 0
EU207658 NADH dehydrogenase subunit 26 2 2 1 8 5 0.000003 0.000006
EU207722 questionable: triacylglycerol lipase 0 0 0 8 0 0 0.000066 0.000002
EU207765 putative: odorant-binding protein G.1A.F 0 0 0 22 0 0 0 0
EU207810 putative: trypsin 0 0 0 21 1 0 0 0
EU207816 hydrogen transporting two sector ATPase 48 8 8 6 8 13 0.000004 0.000001
EU207921 vitellogenin 0 0 0 19 0 0 0 0
EU207965 defensin 17 2 5 0 0 0 0.000002 0.00002
EU208023 questionable: threonine-rich salivary mucin 0 0 0 0 6 4 0.000031 0.000003
EU212979 NADH dehydrogenase 36 5 10 0 4 3 0 0
  1. Clusters and their constituent ESTs were analysed using IDEG6 [20] to find clusters where the number of ESTs were statistically different between the libraries. Of 8,020 clusters analysed, 99 showed a significantly differential number of ESTs collected from one or more of the libraries. Each row represents the Genbank Accession number for cluster, and the columns are the number of ESTs from each of the six libraries that are a member of it. The libraries are: asuhem (bacteria-inoculated, hemocyte), diroinf (whole body, Dirofilaria immitis injected), imacbac (bacteria-inoculated whole body), brumal (blood-fed Brugia malayi), n7 and n14 (naïve, 5–7 and 12–14 days of age). The R value is the inverse log of the Stekel R Score [23], and the Chi value is a general Chi square analysis.