Figure 2

Orthologous human and zebrafish TFRs that contain the miRNA mir-129-2. (A) 20 kb of the human genome (chr11:43,548,001–43,568,000) including the non-genic 13 kb TFR hs11.145 (red bar). Thick blue bars indicate blocks of sequence that are alignable to the orthologous zebrafish TFR dr25.92. Small purple bar indicates the position of the human miRNA mir-129-2. (B) A close up view of 130 bp around mir-129-2, thick purple bar indicates the mature miRNA, thin purple line indicates pre-miRNA hairpin. Blue conservation plot is based on the alignment of 17 vertebrate species and green plot based on pairwise alignment of human and zebrafish that shows a conservation profile consistent with the presence of a miRNA conserved in each species [38]. (C) Syntenic region of the zebrafish genome (20 kb chr25:31,421,001–31,441,000) including the TFR dr25.92. Thick blue bars indicate blocks of sequence that are alignable to the orthologous human TFR hs11.145. Although there are currently no genes annotated in this region, the conservation profile suggests that an ortholog of mir-129-2 resides within the TFR. All images are modified screen shots taken from the UCSC genome browser [31].