Annotations of genes responding to cadmium. The distribution of gene annotations for the list of 45 Daphnia pulex genes (EST clusters) responding to cadmium treatment on the microarray based on results from Blastx searches against the NCBI non-redundant protein database. (A) The assignment of 49 annotations of molecular function to 26 genes from level 3 of the Gene Ontology. (B) The assignment of 74 annotations of biological process to 17 genes from level 4 of the Gene Ontology. Blastx queries recorded the best 5 matches with an E-value threshold of 1 × 10-3 and a minimal value of 33 aligned amino acids. Gene Ontology (GO) terms were assigned to genes using Blast2GO  with the following configurations: Pre-eValue-hit filter 1 × 10-3; Pre-similarity-hit filter 2; Annotation cut-off 35; GO weight 5.