Figure 4

Delaying separation leads to significant changes in gene expression especially in cells of the myeloid lineage. Expression profiles were obtained from RNA samples extracted from cells separated immediately following venesection compared to those separated after a four hour delay on ice. Box plots (A) show the change in gene expression between 0 and 4 hours for independent experiments (I – III) and combined self versus self hybridisation data. The Venn diagrams (B) show the number and overlap between genes showing statistically significant differential expression (as defined in the materials and methods).