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Table 2 Oligonucleotides used to generate serial 5'-deletions of the rat and human HL promoter region.

From: Comparative genomics and experimental promoter analysis reveal functional liver-specific elements in mammalian hepatic lipase genes

Name species and orientationa positionb 5'→3' sequencec
3F r F -1697/-1675 cggaagc TTA GCA GAC AGC GAT TGG C
4F r F -1048/-1030 cggaag CTT GCC TCC TCC TGA GTG C
5F r F -754/-736 cggaagc TTG TCC AGG GCG TCC ATA C
9F r F -211/-196 cggaagctt AGC TTG GCT CAA AAG G
8F r F -86/-71 cggaagctt GTG TTC AAA TAC TGG G
7F r F -75/-58 cggaagct TGG GTA ACA TGT TTT AGG
6F r F -39/-22 cggaag CTT CCA CAA CTA AAT ACC
11F r F -23/-8 cggaagctt CCA AGA AGC ATT CTG G
HHL-685Sac h F -685/-667 ccgagctc TGG TCG CCT TTT CCC TAC C
HHL-306Nhe h F -306/-291 gcatgctagc GAA GCC ACC TAC CCC G
HHL-79Kpn h F -79/-55 ggggtacc TAA CAT GTT GAG AGG
HHL-36Kpn h F -36/-20 ggggtac CAA AGT ATC TAA TAG GC
HHL+13Xba h R +13/-6 gctctaga CTT GGT AAT TTC TGA AGC C
HHL-10Kfw h F -10457/-10437 gtcgac GAA GGA TCA GGT GAG GGA TGG
HHL-10Krev h R -9956/-9975 ggatcc GCT TCA AGG GCA ATG AAA GC
HHL-14Kfw h F -14202/-14183 gtcgac GAC TTG GGG ATA TCC ACA TC
HHL-14Krev h R -13524/-13549 ggatc CTG GTA AAA GGA CAT GAA CAA TAT GG
HHL-22Kfw h F -21929/-21909 gtcgac GAT GGG AAA TGG ACC TAC AGC
HHL-22Krev h R -21543/-21562 ggatcc GAT GAG GAC TGA TTC TCA GC
CATREV2d - R   gca act gac tga aat gcc tc
T3primere - R   att aac cct cac taa ag
  1. a: r and h: rat and human, respectively; F and R: forward and reverse orientation.
  2. b: numbering of the rat and human sequence according to [35] and Ensembl e ! 42 [26], respectively.
  3. c: rat and human HL specific parts of the primer sequences are given in capitals.
  4. d: oligonucleotide specific for pCAT-Basic.
  5. e: oligonucleotide specific for pBluescript.