Table 2 Oligonucleotides used to generate serial 5'-deletions of the rat and human HL promoter region.
Name | species and orientationa | positionb | 5'→3' sequencec | |
|---|---|---|---|---|
3F | r | F | -1697/-1675 | cggaagc TTA GCA GAC AGC GAT TGG C |
4F | r | F | -1048/-1030 | cggaag CTT GCC TCC TCC TGA GTG C |
5F | r | F | -754/-736 | cggaagc TTG TCC AGG GCG TCC ATA C |
9F | r | F | -211/-196 | cggaagctt AGC TTG GCT CAA AAG G |
8F | r | F | -86/-71 | cggaagctt GTG TTC AAA TAC TGG G |
7F | r | F | -75/-58 | cggaagct TGG GTA ACA TGT TTT AGG |
6F | r | F | -39/-22 | cggaag CTT CCA CAA CTA AAT ACC |
11F | r | F | -23/-8 | cggaagctt CCA AGA AGC ATT CTG G |
HHL-685Sac | h | F | -685/-667 | ccgagctc TGG TCG CCT TTT CCC TAC C |
HHL-306Nhe | h | F | -306/-291 | gcatgctagc GAA GCC ACC TAC CCC G |
HHL-79Kpn | h | F | -79/-55 | ggggtacc TAA CAT GTT GAG AGG |
HHL-36Kpn | h | F | -36/-20 | ggggtac CAA AGT ATC TAA TAG GC |
HHL+13Xba | h | R | +13/-6 | gctctaga CTT GGT AAT TTC TGA AGC C |
HHL-10Kfw | h | F | -10457/-10437 | gtcgac GAA GGA TCA GGT GAG GGA TGG |
HHL-10Krev | h | R | -9956/-9975 | ggatcc GCT TCA AGG GCA ATG AAA GC |
HHL-14Kfw | h | F | -14202/-14183 | gtcgac GAC TTG GGG ATA TCC ACA TC |
HHL-14Krev | h | R | -13524/-13549 | ggatc CTG GTA AAA GGA CAT GAA CAA TAT GG |
HHL-22Kfw | h | F | -21929/-21909 | gtcgac GAT GGG AAA TGG ACC TAC AGC |
HHL-22Krev | h | R | -21543/-21562 | ggatcc GAT GAG GAC TGA TTC TCA GC |
CATREV2d | - | R | gca act gac tga aat gcc tc | |
T3primere | - | R | att aac cct cac taa ag | |