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Table 1 Genes differentially expressed depending on genotype and/or ACTH treatment

From: Gene array and real time PCR analysis of the adrenal sensitivity to adrenocorticotropic hormone in pig

Gene symbol Gene name Gene Function p-value (ANOVA) Fold Change
       genotype: MS/LW treatment: ACTH/control
    genotype treatment interaction control ACTH LW MS
Genes up-regulated in Meishan pigs
Rnf2 ring finger protein 2 Transcription regulation 2.81E-13 7.89E-01 1.96E-02 3.5 2.5 1.2 -1.2
Mdk midkine (neurite growth-promoting factor 2) Cell growth/maintenance 4.97E-06 NS NS 2.0 1.5 1.2 -1.1
Snf1lk SNF1-like kinase Protein Kinase activity 8.19E-05 NS NS 1.8 1.8 1.2 1.3
Ccnd1 cyclin D1 Cell growth/maintenance 9.83E-06 NS NS 1.7 1.5 1.1 1.0
Cd99 CD99 molecule Structural/Cell adhesion/ECM 1.01E-05 NS NS 1.7 1.6 1.0 1.0
Col21a1 collagen, type XXI, alpha 1 Structural/Cell adhesion/ECM 9.39E-05 NS NS 1.7 1.5 -1.0 -1.2
Gprc5b G protein-coupled receptor, family C, group 5, member B Signal transduction (glutamatergic) 9.53E-07 NS NS 1.6 1.5 1.1 -1.0
Ldhd lactate dehydrogenase D ATP biosynthesis 3.77E-06 NS NS 1.6 1.6 1.1 1.1
Ppp1r1a protein phosphatase 1, regulatory (inhibitor) subunit 1A Phosphatase inhibitor 8.18E-06 NS NS 1.6 1.4 -1.0 -1.2
Mrps6 mitochondrial ribosomal protein S6 Translation 1.25E-06 NS NS 1.6 1.4 1.1 -1.1
Traf5 TNF receptor-associated factor 5 Signal transduction 5.80E-05 NS NS 1.5 1.5 1.0 1.0
Cse1l CSE1 chromosome segregation 1-like (yeast) Protein transport 2.98E-06 NS NS 1.5 1.4 1.1 1.0
Hist2h2aa histone cluster 2, H2aa4 Nucleosome Assembly 2.10E-05 NS NS 1.5 1.5 -1.1 -1.1
Cd99 CD99 molecule Structural/Cell adhesion/ECM 2.27E-05 NS NS 1.5 1.6 -1.1 1.0
Mif macrophage migration inhibitory factor (glycosylation-inhibiting factor) Cell growth/maintenance 1.25E-05 NS NS 1.5 1.3 1.1 -1.0
Ptpmt1 protein tyrosine phosphatase, mitochondrial 1 Protein Phosphatase activity 1.73E-08 NS NS 1.5 1.5 1.0 1.1
Mdh2 malate dehydrogenase 2, NAD (mitochondrial) Tricarboxylic acid cycle 2.59E-06 NS NS 1.5 1.5 1.0 1.1
Malat1 metastasis associated lung adenocarcinoma transcript 1 (non coding RNA) unknown 4.07E-05 NS NS 1.5 1.3 -1.0 -1.2
Suclg2 succinate-CoA ligase, GDP-forming, beta subunit Tricarboxylic acid cycle 1.82E-05 NS NS 1.4 1.3 1.0 -1.1
Eif3s7 eukaryotic translation initiation factor 3, subunit 7 zeta, 66/67kDa Translation regulation 4.61E-05 5.87E-01 2.64E-02 1.4 1.1 1.1 -1.2
Magi3 membrane associated guanylate kinase, WW and PDZ domain containing 3 Protein Kinase activity 2.40E-05 NS NS 1.4 1.8 -1.2 1.0
Ppap2b phosphatidic acid phosphatase type 2B Protein Phosphatase activity 3.45E-05 6.74E-02 4.48E-02 1.4 2.2 -1.0 1.5
Cys1 cystin 1 unknown 1.08E-08 NS NS 1.4 1.5 -1.1 -1.0
Txnl2 thioredoxin-like 2 Electron transport 5.63E-05 NS NS 1.4 1.3 1.0 1.0
C8orf53 chromosome 8 open reading frame 53 unknown 1.34E-05 NS NS 1.4 1.4 1.1 1.1
C14orf2 chromosome 14 open reading frame 2 unknown 1.65E-05 NS NS 1.4 1.3 1.0 -1.0
Dusp26 dual specificity phosphatase 26 (putative) unknown 3.78E-06 NS NS 1.4 1.4 1.0 1.0
Sod2 superoxide dismutase 2, mitochondrial Super oxide metabolism 1.61E-06 NS NS 1.4 1.5 -1.1 -1.0
Ssr3 signal sequence receptor, gamma (translocon-associated protein gamma) Cotranslational membrane targeting 7.82E-05 NS NS 1.4 1.3 1.1 1.0
Xpnpep1 X-prolyl aminopeptidase (aminopeptidase P) 1, soluble Proteolysis 3.05E-05 NS NS 1.3 1.3 -1.0 -1.0
Hint1 histidine triad nucleotide binding protein 1 Signal transduction 6.24E-05 NS NS 1.3 1.2 1.1 1.0
Hsbp1 heat shock factor binding protein 1 Transcription regulation 6.13E-05 NS NS 1.3 1.2 1.0 -1.0
Mrps10 mitochondrial ribosomal protein S10 Structural constituent of ribosome 6.05E-05 NS NS 1.3 1.3 -1.0 1.0
Lpp LIM domain containing preferred translocation partner in lipoma unknown 4.71E-05 NS NS 1.2 1.3 1.1 1.1
Pgk1 phosphoglycerate kinase 1 Protein Kinase activity 1.83E-05 NS NS 1.2 1.4 -1.1 1.1
Ppap2d phosphatidic acid phosphatase type 2d Protein Phosphatase activity 6.15E-06 NS NS 1.2 1.3 -1.0 1.1
Atp5b ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide ATP biosynthesis 1.07E-05 NS NS 1.2 1.1 1.0 -1.0
Ccny cyclin Y unknown 1.59E-05 NS NS 1.2 1.3 1.0 1.1
Cox6b1 cytochrome c oxidase subunit Vib polypeptide 1 (ubiquitous) Oxydoreductase activity 9.02E-05 NS NS 1.2 1.3 -1.0 1.1
Genes down-regulated in Meishan pigs
C1qa complement component 1, q subcomponent, A chain Cell-cell signaling 3.08E-06 NS NS -2.1 -1.5 -1.1 1.2
Cited1 Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 1 Transcription regulation 1.88E-10 NS NS -1.8 -2.0 1.1 -1.0
Tmem14c transmembrane protein 14C Structural/Cell adhesion/ECM (mito) 4.24E-06 4.81E-01 4.54E-02 -1.8 -1.3 -1.2 1.1
Hmgb3 high-mobility group box 3 Transcription regulation 2.00E-08 NS NS -1.6 -1.4 -1.2 -1.0
Tmem14c transmembrane protein 14C Structural/Cell adhesion/ECM (mito) 5.40E-09 3.37E-01 1.61E-02 -1.6 -1.3 -1.1 1.1
Bphl biphenyl hydrolase-like (serine hydrolase; breast epithelial mucin-associated antigen) Amino acid metabolism 2.71E-06 NS NS -1.5 -1.4 -1.1 -1.0
Ttll12 tubulin tyrosine ligase-like family, member 12 unknown 8.52E-08 NS NS -1.5 -1.6 1.1 -1.0
Gpx3 glutathione peroxidase 3 (plasma) Peroxidase activity 8.06E-08 NS NS -1.5 -1.5 -1.0 -1.1
Fkbp4 FK506 binding protein 4, 59kDa Protein binding 4.64E-05 NS NS -1.4 -1.3 1.0 1.1
Gns glucosamine (N-acetyl)-6-sulfatase (Sanfilippo disease IIID) Glycosaminoglycan catabolism 5.48E-05 NS NS -1.3 -1.5 1.1 -1.1
Nr1h2 nuclear receptor subfamily 1, group H, member 2 Transcription regulation 1.30E-05 NS NS -1.2 -1.3 1.0 -1.1
H2afj H2A histone family, member J Nucleosome Assembly 3.50E-05 NS NS -1.2 -1.3 -1.0 -1.1
Genes up-regulated by ACTH
Bag3 BCL2-associated athanogene 3 Cell growth/maintenance 3.76E-01 9.34E-11 5.19E-03 1.2 -1.3 3.0 2.0
Ier3 immediate early response 3 Cell growth/maintenance 4.49E-01 2.83E-09 3.81E-02 1.3 -1.1 2.6 1.9
Adamts1 ADAM metallopeptidase with thrombospondin type 1 motif, 1 Cell growth/maintenance NS 2.37E-06 NS 1.4 -1.1 2.6 1.7
Gadd45a growth arrest and DNA-damage-inducible, alpha Regulation of protein kinase activity NS 1.46E-07 NS 1.1 -1.1 2.1 1.7
Id3 inhibitor of DNA binding 3, dominant negative helix-loop-helix protein Transcription regulation NS 4.69E-07 NS -1.1 -1.2 2.0 1.9
Tfpi2 tissue factor pathway inhibitor 2 Structural/Cell adhesion/ECM NS 4.72E-07 NS 1.1 1.0 1.8 1.7
Odc1 ornithine decarboxylase 1 Cell growth/maintenance NS 2.85E-06 NS 1.2 1.0 1.6 1.4
Tomm20 translocase of outer mitochondrial membrane 20 homolog (yeast) Protein transport (mb ext mito) NS 9.51E-05 NS 1.2 1.1 1.5 1.4
Timp1 TIMP metallopeptidase inhibitor 1 Cell growth/maintenance NS 2.88E-07 NS 1.0 -1.1 1.4 1.3
Ddx3x DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked RNA helicase activity NS 1.19E-05 NS 1.1 1.0 1.4 1.3
C10orf46 chromosome 10 open reading frame 46 unknown NS 6.70E-06 NS 1.0 1.2 1.4 1.6
Gnl2 guanine nucleotide binding protein-like 2 (nucleolar) unknown NS 3.73E-05 NS 1.1 -1.0 1.3 1.2
Genes down regulated by ACTH
Stat5a signal transducer and activator of transcription 5A Transcription regulation NS 8.12E-05 NS -1.0 1.0 -1.4 -1.3
Wasf2 WAS protein family, member 2 Signal transduction (G-protein) NS 7.81E-05 NS -1.0 1.0 -1.3 -1.3
Unc84b unc-84 homolog B (C. elegans) Cell growth/maintenance NS 4.26E-05 NS 1.2 -1.0 -1.3 -1.6
Cntf ciliary neurotrophic factor Signal transduction NS 4.93E-05 NS -1.0 1.1 -1.3 -1.2
Nfe2l2 nuclear factor (erythroid-derived 2)-like 2 Transcription regulation NS 5.59E-06 NS 1.1 1.0 -1.3 -1.3
Pcbd1 pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (TCF1) Transcription regulation NS 5.70E-05 NS 1.1 1.0 -1.2 -1.3
Bmpr2 bone morphogenetic protein receptor, type II (serine/threonine kinase) Protein Kinase activity NS 3.15E-05 NS -1.1 1.0 -1.2 -1.1
Ctnnb1 catenin (cadherin-associated protein), beta 1, 88kDa Transcription regulation NS 1.01E-05 NS -1.0 -1.1 -1.2 -1.2
Gdf8 growth differentiation factor 8 Signal transduction (TGFb) NS 5.41E-05 NS 1.1 -1.1 -1.2 -1.3
Genes upregulated in Meishan and by ACTH
Crem cAMP responsive element modulator Transcription regulation 4.34E-02 2.70E-10 NS 1.7 1.1 4.4 3.0
Cd83 CD83 molecule Signal transduction 6.77E-06 3.80E-10 NS 1.9 1.5 3.2 2.6
Eif1b eukaryotic translation initiation factor 1B Translation regulation 9.08E-06 7.89E-10 NS 1.5 1.9 2.5 3.3
Tob1 transducer of ERBB2, 1 Transcription regulation 4.11E-02 6.96E-08 NS 1.1 1.0 2.5 2.3
Ldlr low density lipoprotein receptor (familial hypercholesterolemia) Cholesterol mobilization 5.19E-03 4.15E-07 NS 1.6 1.3 2.3 1.8
Chchd2 coiled-coil-helix-coiled-coil-helix domain containing 2 unknown 4.72E-04 3.36E-06 NS 1.4 1.3 1.6 1.5
Ckb creatine kinase, brain Structural/Cell adhesion/ECM 1.03E-06 3.00E-02 NS 2.1 1.9 1.3 1.2
Mki67ip MKI67 (FHA domain) interacting nucleolar phosphoprotein rRNA metabolism 1.25E-04 9.14E-05 NS 1.3 1.3 1.3 1.3
Maob monoamine oxidase B Electron transport 4.43E-05 4.23E-03 NS 1.4 1.3 1.2 1.2
Dnaja DnaJ (Hsp40) homolog, subfamily A, member 2 Cell growth/maintenance 1.59E-05 2.55E-03 NS 1.5 1.2 1.2 1.0
H19 H19, imprinted maternally expressed untranslated mRNA unknown 9.02E-07 5.79E-03 NS 1.5 1.6 1.1 1.3
Dleu1 deleted in lymphocytic leukemia, 1 Cell growth/maintenance 4.90E-07 2.14E-03 NS 1.5 2.0 1.1 1.4
Genes up-regulated in Meishan and down-regulated by ACTH
Selenbp1 selenium binding protein 1 Transporter activity 2.98E-05 1.41E-02 NS 1.3 1.5 -1.3 -1.1
C16orf33 chromosome 16 open reading frame 33 unknown 5.03E-05 3.98E-02 NS 1.2 1.3 -1.2 -1.0
Ech1 enoyl Coenzyme A hydratase 1, peroxisomal Fatty acid metabolism 5.27E-07 6.31E-03 NS 1.4 1.4 -1.2 -1.2
Anpep alanyl (membrane) aminopeptidase (aminopeptidase N, aminopeptidase M, microsomal aminopeptidase, CD13, p150) Development/Morphogenesis 5.65E-12 8.27E-03 NS 2.1 1.9 -1.1 -1.2
Iscu iron-sulfur cluster scaffold homolog (E. coli) unknown 6.09E-05 1.03E-03 NS 1.3 1.2 -1.1 -1.2
Enpp2 ectonucleotide pyrophosphatase/phosphodiesterase 2 (autotaxin) Signal transduction (G-protein) 8.22E-05 6.17E-03 NS 1.4 1.3 -1.1 -1.2
Klhl22 kelch-like 22 (Drosophila) unknown 2.75E-04 9.94E-05 NS 1.3 1.1 -1.1 -1.2
Sdha succinate dehydrogenase complex, subunit A, flavoprotein (Fp) Tricarboxylic acid cycle 3.38E-05 3.08E-02 NS 1.2 1.2 -1.0 -1.1
Genes down regulated in Meishan and by ACTH
Ctdsp2 CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 2 unknown 1.84E-02 4.68E-08 NS -1.1 -1.3 -1.6 -1.8
Pecam1 platelet/endothelial cell adhesion molecule (CD31 antigen) Signal transduction 2.74E-05 8.45E-04 NS -1.6 -1.6 -1.4 -1.5
Dab2 disabled homolog 2, mitogen-responsive phosphoprotein (Drosophila) Cell growth/maintenance 2.92E-02 5.63E-05 NS -1.1 -1.3 -1.4 -1.7
Cyb5b cytochrome b5 type B (outer mitochondrial membrane) Transporter activity 4.15E-03 2.59E-06 NS -1.1 -1.3 -1.4 -1.6
Ostf1 osteoclast stimulating factor 1 Signal transduction 1.66E-07 1.08E-02 NS -1.7 -1.5 -1.3 -1.1
Gdf8 growth differentiation factor 8 Signal transduction (TGFb) 4.95E-03 6.71E-05 NS -1.1 -1.3 -1.3 -1.4
Nfat5 nuclear factor of activated T-cells 5, tonicity-responsive Transcription regulation 2.75E-03 1.27E-05 NS -1.2 -1.2 -1.3 -1.3
Acox1 acyl-Coenzyme A oxidase 1, palmitoyl Fatty acid metabolism 7.62E-10 4.31E-04 NS -1.7 -1.5 -1.2 -1.1
Ephx1 epoxide hydrolase 1, microsomal (xenobiotic) Proteolysis 1.60E-06 2.62E-02 NS -1.8 -1.6 -1.2 -1.1
Ccdc85b coiled-coil domain containing 85B unknown 1.66E-05 4.54E-02 NS -1.6 -1.4 -1.2 -1.1
A2m alpha-2-macroglobulin Protein transport 2.45E-10 3.68E-03 NS -1.8 -1.7 -1.2 -1.1
Nono non-POU domain containing, octamer-binding mRNA processing 1.01E-06 3.80E-03 NS -1.4 -1.3 -1.2 -1.1
Ifltd1 intermediate filament tail domain containing 1 unknown 6.75E-06 1.81E-04 NS -1.2 -1.4 -1.2 -1.3
Myst4 MYST histone acetyltransferase (monocytic leukemia) 4 Transcription regulation 9.48E-06 1.18E-02 NS -1.3 -1.3 -1.1 -1.2
Genes down regulated in Meishan and up-regulated by ACTH
Gadd45b growth arrest and DNA-damage-inducible, beta Regulation of protein kinase activity 6.38E-04 1.41E-11 NS -1.3 -1.3 2.4 2.4
C14orf4 chromosome 14 open reading frame 4 unknown 8.66E-03 7.41E-05 NS -1.0 -1.2 1.4 1.2
  1. Genes differentially expressed between Meishan (MS) and Large White (LW) pigs and/or in response to acute stimulation by ACTH were identified using a pig cDNA microarray made up of 8959 cDNA clones. The most significant affected genes (p < 0.0001) were listed in the table, categorized on the basis of up or down-regulated by genotype and/or ACTH and sorting by decreasing fold changes. (unknown transcripts were excluded) Complete list is given in the additional file 1.