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Table 1 List of the EST database of Nilaparvata lugens

From: Annotated ESTs from various tissues of the brown planthopper Nilaparvata lugens: A genomic resource for studying agricultural pests

Library Tissue/sex/stage Vector used No. of clones Average length (bp) %"N" No. of clusters
AA Abdomen, female, 0–1 day ZAP 609 589.7 0.014 435
AB Abdomen, female, 0–1 day ZAP 1027 601.9 0.011 667
AC Abdomen, male, 0–1 day ZAP 588 599.7 0.022 493
AD Abdomen, male, 0–1 day ZAP 967 594.9 0.102 726
EA Egg, 0–1.5 day pGEM 3726 652.0 0.051 2371
EB Egg, 3–5 day pGEM 2055 576.3 0.076 941
HA Head, female, 0–1 day ZAP 777 520.6 0.018 111
HT Head-thorax, male, 0–1 day ZAP 2555 673.9 0.070 1132
MA Midgut, female, 0–5 day pGEM 3594 637.5 0.051 1252
MB Midgut, male, 0–5 day pGEM 2095 606.4 0.117 847
NA Whole nymph, 1st instar pGEM 3269 633.8 0.096 1714
NB Whole nymph, 4th instar ZAP 3710 624.7 0.058 1749
OA Ovary, 0–1 day ZAP 967 511.5 0.119 689
OB Ovary, 4–5 day ZAP 822 601.9 0.028 329
OC Ovary, 4–5 day ZAP 2225 604.4 0.079 989
SG Salivary glands, female, 0–5 day pGEM 2383 640.8 0.083 723
TA Testis, 0–1 day ZAP 1525 575.9 0.045 895
TH Thorax, 0–1 day ZAP 4228 693.1 0.029 1657
Total    37122 626.7 0.065 10261
  1. ZAP, lambda ZAP II (pBluescript) phage vector; pGEM, pGEM-t plasmid vector; %"N", percentage of ambiguous nucleotides. Ovary and testis includes the accessory glands of the reproductive organs. Clustering was performed using CLOBB [25]. ESTs are deposited in the DNA databases under accession numbers DB20956–DB858077 (37,122 entries).