Rotary model for E. coli F
ATPase and variation in the mammalian ATP6 subunit. A) Rotary model for E. coli F1F0 ATPase (see text for details); B) Topological assignment of the sites that present a high number of strong positively selected amino acid properties under positive-destabilizing selection in ATP6 (corresponds to the a subunit in E. coli). The transmembrane domains location is shown in grey (for details see Material and Methods section). The dark grey domain was only predicted by one of the three methods used.