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Table 2 Primers used to perform the genomic DNA amplification. Additional couples of primers (e.g. 1N, 1NN and 2-3N) were used for each amplicon as it was not possible to amplify DNA from all the primates using a single set (e.g. 1 and 2–3).

From: Evolution of the hepcidin gene in primates

EXON

FORWARD PRIMER

REVERSE PRIMER

1

5' tctctcccgccttttcgg 3'

5' tgaggcctggctctccc 3'

1N

5' gccttttcggcgccac 3'

5' agacgtcctgagctctgctca 3'

1NN

5' ccccataaaagcgactgtcac 3'

5' ctcccatccctgctgcc 3'

2–3

5' gtttaaaccacttggagaggagca 3'

5' acactcggcagagagaaaggac 3'

2-3N

5' gaggtccactgggcccc 3'

5' acatgacccaccaagcactg 3'