Figure 5

Phylogenetic tree of Hog domains. Phylogenetic trees were built from aligned Hog domains (Figure 2 – 4). The Neighbor joining tree was created using the default settings of Clustal_X. Bootstrap values of 1000 trials are indicated in the figure. In this and subsequent phylogenetic tree figures Enoplea sequences are highlighted in light green, Cnidarian sequences in yellow, Choanoflagellate sequences in light red and fungal sequences in blue. The Hh sequences are marked with Hh and the nematode Hh-related sequences are marked with NemaHog. The root was placed between the red algae/plant sequences and the remaining sequences. Some incomplete sequences were omitted in this tree. Additional phylogenetic analyses were also carried out, for example by omitting the protist sequences and using the fungal sequence GmGIN1 as outgroup (Additional files 5, 6, 7). Overall, the results were very similar.