Figure 5

Strand bias with respect to A+/T+ segment boundaries – comparison with no recorded transcription. The orange line shows the AT-bias for the entire sample. The red line shows the AT-bias for the boundaries where there is no recorded transcription from coding genes for 50 kb either side of the boundary – this line is based on 4997 segment boundaries. The blue line shows the AT-bias for the boundaries where there is some recorded transcription from coding genes within the range plotted – this line is based on 6753 segment boundaries. The thickness of the red and blue lines show 95% confidence limits. All lines show moving averages over 100 bases. There is a statistically significant difference between the red and blue lines: at position 5000 bases downstream of the boundary a two-tailed z-test gives p-value < 10^-50, n1 = 4997, n2 = 6753, z = -16.