From: Analysis of Aspergillus nidulans metabolism at the genome-scale
Functional annotation (A) | |
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Comparison of the previous functional annotations for the candidate ORFs [5] with the function of interest | |
A | function assigned to the candidate ORF consistent with function of interest |
A* | function assigned to the candidate ORF not contradicting function of interest (e.g. aldose reductase versus xylose reductase; mitochondrial isocitrate dehydrogenase versus cytosolic isocitrate dehydrogenase) |
A-NA | no function assigned to the candidate ORF (e.g. hypothetical protein) |
A-X | function assigned to the candidate ORF not consistent with function of interest |
Protein families (F) | |
Comparison of the protein domains originally predicted for the candidate ORF [5] with those of the enzymes catalysing the function of interest [6] | |
F | protein family of candidate ORF consistent with protein family of function of interest |
F-NA | no protein family associated to the candidate ORF |
F-X | protein family of candidate ORF not consistent with protein family of function of interest |
Comparative genomics with yeast (Y) | |
BLASTP searches of all proteins in S. cerevisiae [35] against all predicted proteins in A. nidulans [5] | |
Y | function of the protein in yeast (that has a match with the candidate ORF) consistent with missing function |
Y* | function of the protein in yeast (that has a match with the candidate ORF) not contradicting missing function (e.g. aldose reductase versus xylose reductase; mitochondrial isocitrate dehydrogenase versus cytosolic isocitrate dehydrogenase) |
Y-NA | no match of protein in yeast with the candidate ORF |
Y-X | function of the protein in yeast (that has a match with the candidate ORF) not consistent with missing function |
Comparative genomics with other organisms (O) | |
BLASTP searches of candidate ORF in A. nidulans against (non-redundant) protein database at NCBI [26] | |
O | functions of proteins in other organisms (that have a match with the candidate ORF) consistent with missing function |
O* | functions of proteins in other organisms (that have a match with the candidate ORF) not contradicting missing function (e.g. aldose reductase versus xylose reductase; mitochondrial isocitrate dehydrogenase versus cytosolic isocitrate dehydrogenase) |
O-NA | no matches of candidate ORF with proteins in other organisms |
O-X | functions of proteins in other organisms (that have a match with the candidate ORF) not consistent with missing function |