Figure 5

Silencing of the transcribed pseudogene ABCC6P1 downregulates mRNA levels of ABCC6. Targeted siRNA-mediated inhibition of ABCC6P1 expression in HepG2 cells results in significant downregulation (69%, 95%-CI: 65–73%) of the pseudogene transcript levels (lane 3). mRNA levels are normalized to β-actin (yellow bars) and cyclophilin B (blue bars). ABCC6P1 silencing is associated with a reduction of ABCC6 mRNA expression (84%, p = 0.003, 95%-CI: 76–91%, yellow bar; 82%, p = 0.01, 95%-CI: 72–93%, blue bar, lane 4). A positive control (knock-down of GAPDH) is shown (lane 2). Data were obtained from six independent experiments. The relative expression levels before knock-down experiments were set to 100% (lane 1).