Figure 5

Clock-gene expression profiles in selected transcription factor misexpression lines. Seedlings from ox- and wild-type plants were harvested every 4 hours. Total RNA was the substrate for RT-PCR against the coding regions of the core-clock genes CCA1, LHY, GI, or TOC1, and as a control, TUBULIN (TUB). Results are presented as proportional to the average value after normalization with respect to TUB. Expression profiles in the control, MYB3R2-ox and bHLH69-ox were represented as blue, orange, and pink lines, respectively. (A) LHY, (B) TOC1, (C) CCA1, and (D) GI.