Figure 5

Inversions and rearrangements in PXO99^A compared to MAFF. The alignment shows regions of PXO99^A that align to the same (red) or opposite (blue) strand of MAFF. Transposase genes and their orientation (+ or -) are shown at the sites of each rearrangement. Letters A-J indicate specific rearrangement events. A: the IS element ISXoo3 is composed of two distinct and independently conserved ORFs and is responsible for an inversion spanning coordinates 267869–5114959 (all coordinates refer to the PXO99^A genome). B: ISXo8 occurs in opposite orientation at each end of a 2.6 Mbp inversion spanning positions 1356757–3898472. C: ISXo1 occurs in inverted copies at the endpoints of a 1.8 Mbp inversion spanning 1558996–3391786. D: a 33270 bp inverted region spanning 4394742–4428012 is flanked by oppositely-oriented copies of ISXo8. E: Each copy of the 212-kb duplication is flanked by ISXo5, which also occurs adjacent to two other translocations in this region. The duplication appears as two parallel diagonal lines in this box. F: ISXo8 also occurs in inverted copies at the boundaries of a 47540 bp segment that is translocated from approximately 4800000 to 685272. G: ISXoo3 flanks both ends of a 47540 bp translocation from approximately 1117000 to 4339239. H: A 9,862 bp region occurs in inverted copies at 217,455 and 4,305,307. MAFF311018 contains only one copy of this region. I,J: Segments spanning 96,753 bp (I) and 17,021 bp (J) are inverted with respect to MAFF311018 but not associated with transposases.