Figure 9

Quantitative real-time PCR analysis of selected mRNAs in human gastrointestinal tissues. Genes were selected for analysis from those that showed differential crypt-villus expression by microarray analysis, including mRNA encoding HD5, Reg3A, aldolase-B, Eps8L2, Muc3β, semaphorin3F, RETLNB, ApoA4, Eph-Rb3 and Pdxp. The mRNA copy counts were determined from standard curves using gene-specific plasmids and are expressed as a fraction relative to measure values for β-actin. Ileal samples (n = 5) and colon (n = 5) were from non-diseased surgical samples of adjoining tissue resected for bowel obstruction or colon cancer. Single samples of commercially obtained (pooled) RNA were analyzed for the other tissues. Error bars represent standard deviations from the geometric mean. For single specimen samples (E, D, J) inter-assay variation was <15% for mRNA copy counts ≥10 exp4/10 ng RNA, and 40% for mRNA copy counts ≤10 exp2/10 ng RNA. E, esophagus; D, duodenum. J, jejunum; I, ileum; C, colon.