Table 2 Independent confirmation of the yeast two-hybrid results
Interaction in two-hybrid assay | Confirmation method | Reference |
|---|---|---|
CPSF160 – CPSF100 | In vitro pull-down assay; Co-purification from nuclear extracts with tagged protein | [31, 34] |
CPSF100 – CPSF73-I | In vitro pull-down assay; Co-purification from nuclear extracts with tagged protein | [31, 34] |
CPSF100 – CPSF73-II | In vitro pull-down assay; Co-purification from nuclear extracts with tagged protein | [31, 34] |
CPSF100 – CPSF30 | In vitro pull-down assay | [34] |
CPSF30 – CPSF30 | In vitro copurification with recombinant proteins | [27] |
CPSF100 – FY | Co-purification from nuclear extracts with tagged protein | [31] |
CPSF30 – FIPS5 | In vitro copurification with recombinant proteins, direct effects on enzyme activity | [28, 29] |
CSTF77 – CSTF64 | In vitro copurification with recombinant proteins | [30] |
CSTF77 – FIPS5 | In vitro copurification with recombinant proteins | [29] |
CFIS1 – FIPS5 | In vitro copurification with recombinant proteins | [29] |
FIPS5 – PABN2 | In vitro copurification with recombinant proteins | [29] |
FIPS5 – PAPS4 | In vitro copurification with recombinant proteins, direct effects on enzyme activity | [29] |
CLPS3 – PCFS1 | In vitro pull-down | D. Xing and QQ. Li, unpublished data (manuscript in prep) |
CLPS3 –PCFS5 | In vitro pull-down | D. Xing and QQ. Li, unpublished data (manuscript in prep) |
CLPS3 – PCFS4 | Affinity co-purification | [39] |