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Table 2 Independent confirmation of the yeast two-hybrid results

From: Arabidopsis mRNA polyadenylation machinery: comprehensive analysis of protein-protein interactions and gene expression profiling

Interaction in two-hybrid assay Confirmation method Reference
CPSF160 – CPSF100 In vitro pull-down assay; Co-purification from nuclear extracts with tagged protein [31, 34]
CPSF100 – CPSF73-I In vitro pull-down assay; Co-purification from nuclear extracts with tagged protein [31, 34]
CPSF100 – CPSF73-II In vitro pull-down assay; Co-purification from nuclear extracts with tagged protein [31, 34]
CPSF100 – CPSF30 In vitro pull-down assay [34]
CPSF30 – CPSF30 In vitro copurification with recombinant proteins [27]
CPSF100 – FY Co-purification from nuclear extracts with tagged protein [31]
CPSF30 – FIPS5 In vitro copurification with recombinant proteins, direct effects on enzyme activity [28, 29]
CSTF77 – CSTF64 In vitro copurification with recombinant proteins [30]
CSTF77 – FIPS5 In vitro copurification with recombinant proteins [29]
CFIS1 – FIPS5 In vitro copurification with recombinant proteins [29]
FIPS5 – PABN2 In vitro copurification with recombinant proteins [29]
FIPS5 – PAPS4 In vitro copurification with recombinant proteins, direct effects on enzyme activity [29]
CLPS3 – PCFS1 In vitro pull-down D. Xing and QQ. Li, unpublished data (manuscript in prep)
CLPS3 –PCFS5 In vitro pull-down D. Xing and QQ. Li, unpublished data (manuscript in prep)
CLPS3 – PCFS4 Affinity co-purification [39]