Figure 1

Phenotypic analysis of T-cell ex vivo activation upon anti-CD3/anti-CD28 stimulation of three populations. Three independent biological experiments were carried out for each population. CD3+, CD4+ and CD8+ T cells were negatively selected from PBMCs of healthy donors and activated with anti-CD3/anti-CD28 antibodies. (A) T-cell expansion as assessed by cell numbers; (B) The percentage of the viable T cells as determined by flow cytometry; (C) The percentage of the viable cells expressing CD69; (D) The percentage of the viable cells expressing CD25. Data from 6 independent experiments (CD3+ T-cell experiments, E1–E3, and CD4+ and CD8+ T-cell experiments, E7–E9) using cells from 6 different healthy donors are shown.