Table 2 Fluorescence induction of P. luminescens carrying different promoter-reporter gene fusions in presence of insect larvae homogenate
From: Photorhabdus luminescens genes induced upon insect infection
Promoter of gene/operon | Putative function of the gene product(s) | Fluorescence Induction |
|---|---|---|
Toxins | ||
tccC1 (plu4167) | insecticidal toxin complex TccC1 | 10.2-fold (± 2.0) |
plu1645 | similar to photopexin A und B (hemopexin-domain) | 10.1-fold (± 4.5) |
plu2400 | homologous to C-terminal region of dermonecrotic toxin (DNT) of Pasteurella multocida | 5.5-fold (± 1.9) |
plu4122 | contains Fascin domain, function unknown | 6.3-fold (± 2.8) |
Metabolism | ||
agaZSVCD/plu0839/gatY |  | 3,3-fold (± 0.3) |
   agaZ (plu0833) | tagatose-6-phosphate-Kinase |  |
   agaS (plu0834) | tagatose-6-phosphate ketose/aldose isomerase |  |
   agaV (plu0835) | PTS-system, N-acetylgalactosamine-specific IIB component 2 (EIIB-AGA') |  |
   agaC (plu0836) | PTS-system, N-acetylgalactosamine-specific IIC component 1 (EIIC-AGA) |  |
   agaD (plu0837) | PTS-System, N-acetylgalactosamine-specific IID component (EIID-AGA) |  |
   plu0838 | unknown, putative PTS permease |  |
   gatY (plu0839) | tagatose-bisphosphate aldolase GatY |  |
eutABC |  | 3.5-fold (± 1.0) |
   eutA (plu2969) | ethanolamine degradation |  |
   eutB (plu2970) | ethanolamine-ammonia-lyase heavy chain |  |
   eutC (plu2971) | ethanolamine-ammonia-lyase light chain |  |
hutUH |  | 2.5-fold (± 1.1) |
   hutU (plu3193) | urocanate hydratase (urocanase) |  |
   hutH (plu3192) | histidine ammonia lyase (histidase) |  |
sucABCD |  | 1.5-fold (± 0.4) |
   sucA (plu1430) | α-oxoglutarate dehydrogenase E1 component |  |
   sucB (plu1431) | dihydrolipoamid succinyltransferase component of α-oxoglutarate dehydrogenase complex (E2) |  |
   sucC (plu1432) | succinyl-CoA synthetase β chain |  |
   sucD (plu1433) | succinyl-CoA synthetase α-chain |  |
plu1864 | similarities to phosphoenolpyruvat phosphomutase | 1.0-fold (± 0.3) |
plu2604 | similar to glutarredoxin Protein YdhD | 5.0-fold (± 1.9) |
Regulation | ||
mlc (plu2226) | „making large colonies" protein, involved in regulation of sugar metabolism | 4.8-fold (± 1.7) |
DNA-modification | ||
isplu16A (plu3160) | transposase, IS630 family | 5.1-fold (± 1.0) |
plu1165 | putative relaxase | 5.5-fold (± 2.0) |
plu3688 | putative integrase/recombinase | 7.5-fold (± 3.1) |
Synthesis of cell structure | ||
spr (plu2864) | lipoprotein spr precursor | 3.0-fold (± 1.0) |
plu2790 | similar to N-acetylmuramoyl-L-alanine amidase YbjR precursor of Escherichia coli | 4.7-fold (± 1.3) |
plu1463 | similar to DNA inversion product and tail-fiber protein of lambdoid prophage | 6.5-fold (± 1.9) |
Transport, antibiotic resistance | ||
plu4229 | multidrug-resistance permease | 2.4-fold (± 0.7) |
plu1579 | similar to bacitracin permease of E. coli | 4.4-fold (± 1.4) |
Antibiotic synthesis | ||
plu3567-3561 |  | 3.8-fold (± 1.2) |
   plu3567 | similar to N-formimidoyl fortimicin A synthase |  |
   plu3566 | similar to putative methylase and protoporphyrinogen oxidase |  |
   plu3565 | similar to class II aminotransferase and 5-aminolevulinic acid synthase |  |
   plu3564 | weakly similar to PapB protein and to chorismate mutase/prephenate dehydrogenase |  |
   plu3563 | similar to p-aminobenzoic acid synthase |  |
   plu3562 | similar to dehydrogenase PapC of Streptomyces pristinaespiralis |  |
   plu3561 | probable transport protein |  |
Unknown function | ||
plu0801 | PrK012399-domain, similar to Plu1012 and Plu1017 | 2.0-fold (± 0.3) |
plu1012-1010 |  | 10.6-fold (± 4.2) |
   plu1012 | PrK012399-domain, similar to Plu0801 and Plu1017 |  |
   plu1011 | „TIM-br_sig_trns"-Domain, putative sigma54-dependent transcriptional activator |  |
   plu1010 | unknown |  |
plu1017 | PrK012399-domain, similar to Plu0801 and Plu1012 | 6.6-fold (± 4.6) |
plu1672 | similar to Plu1691 of Photorhabdus, function unknown | 2.0-fold (± 0.8) |
plu1950 | similar to Plu2538 of Photorhabdus, function unknown | 3.0-fold (± 0.9) |
plu2652 | function unknown | 4.0-fold (± 1.5) |
plu2809 | similar to protein YcfD of E. coli, contains JmjC domain (pot. metalloenzym) | 3.0-fold (± 1.1) |
plu3479 | unknown | 1.1-fold (± 0.2) |
plu3608 | similar to protein Plu3387 of Photorhabdus, function unknown | 4.5 (± 0.3) |
Controls | ||
mcf (plu4142) | "makes caterpillars floppy" toxin | 6.0 (± 2.3) |
mcf2 (plu3128) | "makes caterpillars floppy" toxin 2 | 0.9 (± 0.2) |
tcdA1 (plu0962) | insecticidal toxin complex TcdA1 | 6.5 (± 1.0) |
tcaA/B (plu0516) | insecticidal toxin complex TcaA/TcaB | 1.25 (± 0.2) |
tcaZ (plu0514) | insecticidal toxin complex TcaZ | 1.3 (± 0.3) |
tccA1 (plu4169) | insecticidal toxin complex TccA1 | 1.6 (± 0.2) |
prtA (plu0655) | alkaline metalloprotease PrtA | 2.1-fold (± 0.7) |