Figure 4From: Genomic analysis of post-mating changes in the honey bee queen (Apis mellifera)Analysis of Ovary Gene Expression. a. Principal components analysis of transcript profiling in the ovaries demonstrated similar patterns as the brain. The first principal component accounts for 83% of the variation, and shows little differences among groups. The second principal component accounts for 10% of the overall variation and demonstrates that mated queens represent an extreme phenotype. The final principal component explains 7% of the variation, and identifies laying queens as the outgroup. b. Hierarchical clustering of the 50 most-predictive transcripts in the ovaries reveals that mated and laying queens have transcriptional profiles more similar to each other than to virgins. This grouping is supported by an "approximately-unbiased" p-value of 73 and a boostrap value of 64, and reflects the same patterns observed in ovary development and vitellogenin levels.Back to article page