Figure 3From: Sequence polymorphism can produce serious artefacts in real-time PCR assays: hard lessons from Pacific oystersOutcomes of level of cDNA expression across families for primers (3, 5, 6, 7, 8, 9) technically validated through the analysis of the melting curve of the EST [ AJ565694]. From left to right: Plot of PCR cycle number against logarithm PCR product amount; Derivative Melting Curve; Statistical results of level of cDNA expression across families based on a multiple comparison test (Tukey). Family numbers are reported on the horizontal axis, histograms sharing a letter are not significantly different (P < 0.05).Back to article page