A novel resource for genomics of Triticeae: BAC library specific for the short arm of rye (Secale cereale L.) chromosome 1R (1RS)

Table 1 Results of 1RS BAC library screening on pools of 384-well plates with the 1RS-specific DNA markers

Marker code	Reference	Primers	Number of positive pools
SSR markers
scm9	Saal and Wricke 1999	TGACAACCCCCTTTCCCTCGTTCATCGACGCTAAGGAGGACCC	10
STS markers*
bcd1434 ¹	Loarce et al. 1996	CCACCTTCTCGCTGTTGAAT CTGCTGTCCAGCCAGAAAAT	11
mwg2062 ²	Korzun et al. 1998	TCTCGCTGGTATTCAGGGTCC AAACGATAGCAAGAGGAACCG	11
psr109 ³	Masojc et al. 2001	GACTACCTGATCCGCTCCAA ACTTGCACTTTAGGCCTTGC	6
psr949 ⁴	Masojc et al. 2001	GCCCTGCCATATAACTTCCA GCATCATGTCTCCCTTTACCA	16
IB-262	Mago et al. 2002	GTAGGTAATGTATCAGAGTTGTAC GTCTTTGTGCTCGGTAGCTCC	14
Average			11.3

* developed from RFLP markers
¹ primers designed from bcd1434-3 sequence (GrainGenes http://wheat.pw.usda.gov/GG2/index.shtml)
² primer sequences downloaded from GrainGenes http://wheat.pw.usda.gov/GG2/index.shtml
³ primers designed from psr109 sequence (GrainGenes http://wheat.pw.usda.gov/GG2/index.shtml)
⁴ primers designed from psr949-Forward sequence (GrainGenes http://wheat.pw.usda.gov/GG2/index.shtml)

ISSN: 1471-2164