Figure 3

Expression of CD41 and CD144 in embryoid bodies. Immunohistochemical analysis of CD41 and CD144 staining of frozen embryoid body sections (see methods for details). (A) Evaluation of CD41 (green) and CD144 (red) staining by immunofluorescence in embryoid body at day 6.5. Overlapping images of CD41 and CD144 demonstrate spatial proximity of CD41/CD144 expression. DAPI nuclear staining (blue) outlines the general embryoid body morphology and organization. Bar represents 300 μm. (B) Immunofluorescence staining for vWF (green), VE-Cadherin (red), and DAPI (blue) in embryoid bodies at day 10.5. Colocalization of the two endothelial-specific markers, vWF and CD144, is evident in the merged images. Bar represents 200 μm.