Figure 3
From: Exploiting orthologue diversity for systematic detection of gain-of-function phenotypes
Xenoarray analysis on MDCK cells. (A) Dot plot comparing the signal intensity for GFP-transduced and library-transduced cells, as indicated. Murine transcripts, specifically detected by the murine microarray only in the transduced cells, are highlighted by a continuous red circles. (B) Growth curve on control and selected populations. The x-axis shows the time, the y-axis the percent of viable cells number, compared to 100% at day 0. The data represent the mean and standard deviation of triplicate values. (C) Soft agar growth on control and selected populations. Segmentation of single colonies and analysis of the relative diameters were performed by the Attovision 1.5 software (BD biosciences) on images captured by a BD Pathway system (BD biosciences). Briefly, a threshold of colony size was defined as the 95th percentile of the colony size in pictures taken from the GFP-UNS cells. Then the number of colonies above the threshold was calculated for all fields (8 fields/sample). The bar chart indicates the average and standard error of the number of colonies/field above the threshold for the GFP- or library-transduced cells, unselected or selected on polyhema, as indicated. (D, E) Xenoarray analysis on library-transduced MDCK cells before (x-axis) and after (y-axis) selection, performed on first infection (D) and second infection (E). The red circles indicate the area grossly corresponding to transcripts enriched in selected cells.