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Table 1 Transcript data of adult mouse retina obtained with high throughput technologies

From: Validation of oligoarrays for quantitative exploration of the transcriptome

  Oligoarrays MPSS SAGE
All detected genes    
No. of unique transcripts1 9,786 6,088 4,827
Total transcript concentration2 1.1·1011 (no. per μg total RNA) 5.6·105 (tpm) 7.6·105 (tpm)
Average transcript concentration (range)3,4 1.1·107 (3.1·104 – 1.4·109) (no. per μg total RNA) 92 (1 – 22,008) (tpm) 77 (18 – 6,480) (tpm)
Total no. transcripts per cell2,3 1.1·106 2.8·105 3.8·105
Average no. of transcripts per cell and gene (range)3,4 112 (0.3 – 14,387) 46 (0.5 – 11,004) 38 (9 – 3,240)
Overlapping genes    
No. of unique transcripts1 1,777 1,777 1,777
Total transcript concentration2 3.8·1010 (no. per μg total RNA) 2.2·105 (tpm) 1.5·105 (tpm)
Average transcript concentration (range)3,4 2.1·107 (1.6·105 – 9.7·108) (no. per μg total RNA) 126 (1 – 6,360) (tpm) 86 (18 – 2,536) (tpm)
Total no. transcripts per cell2,3 3.8·105 1.1·105 7.6·104
Average no. of transcripts per cell and gene (range)3,4 213 (1.6 – 9,705) 63 (0.5 – 3,180) 43 (9 – 1,268)
  1. 1 Mapped to UniGene identifiers.
  2. 2 Sum for all genes.
  3. 3 10 pg total RNA per cell was assumed for oligoarrays, and a total number of 5·105 transcripts per cell was applied for SAGE and MPSS in the calculation.
  4. 4 Average and range for all genes.