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Table 2 Transcript concentration of selected genes in adult mouse retina1

From: Validation of oligoarrays for quantitative exploration of the transcriptome

UniGene ID Gene Symbol Gene name Oligoarray MPSS SAGE qRT-PCR
Genes with high expression
Mm.371592 Ubb Ubiquitin B 9705 401 435 NA
Mm.368524    8916 3180 722 NA
Mm.47709 Pdc Phosducin 6027 516 425 10.36
Mm.151562 Rbp3 Retinol binding protein 3 5733 1292 657 4.77
Mm.16224 Guca1a Guanylate cyclase activator 1a (retina) 5366 611 546 0.66
Mm.284811 Unc119 Unc-119 homolog (C.elegans) 4812 335 1046 2.40
Mm.59151 Guca1b Guanylate cyclase activator 1B 4478 3116 287 1.44
Mm.235863    4046 1312 361 NA
Mm.285993 Calm1 Calmodulin 1 3304 543 222 3.09
Mm.28643 Vamp2 Vesicle-associated membrane protein2 3250 683 296 2.63
Mm.16831 Ckb Creatine kinase, brain 2896 1164 620 NA
Mm.223674 Syp Synaptophysin 2592 595 130 0.16
Mm.1372 Pde6b Diesterase 6B, cGMP, rod reseptor, beta polypeptide 2400 956 982 1.73
Mm.156506 Pcdh21 Protocadherin 21 2137 570 232 1.80
Mm.297482 Tpt1 Tumor protein, translationally controlled 1 1742 359 750 1.44
Mm.3667 Vtn Vitronectin 1630 634 398 1.74
Mm.352239 Gpsn2 Glycoprotein, synaptic 2 1579 484 287 0.02
Mm.55143 Dkk3 Dickkopf homolog 3 (Xenopus laevis) 1576 266 185 0.10
Mm.726 Bsg Basigin 1490 870 268 0.87
Mm.683    1472 236 407 NA
Mm.28147 Reep6 Receptor accessory protein 6 1394 1229 445 3.42
Mm.273538   cDNA, clone Y1G0119M19 1389 375 259 NA
Mm.16228 Slc25a4 Solute carrier family 25, member 4 1382 520 416 0.67
Mm.235204 Atp1b2 ATPase, Na+/K+ transporting, beta 2 polypeptide 1210 466 213 1.79
Mm.94160 Bex2 Brain expressed X-linked 2 1171 339 139 0.09
Mm.331 Ubc Ubiquitin C 1165 423 130 NA
Mm.26633 Plekhb1 Pleckstrin homology domain containing, family B member 1 931 474 620 0.32
Mm.305152 Apoe Apolipoprotein E 920 1046 213 0.25
Mm.200608 Clu Clusterin 885 456 222 0.07
Mm.278865 Stxbp1 Syntaxin binding protein 1 873 512 389 0.60
Mm.236513 Pcbp2 Poly(rC) binding protein 2 777 401 325 1.78
Mm.41926    698 271 130 NA
Mm.373613    661 340 268 NA
Mm.379381    650 261 278 NA
Mm.197518 Laptm4b Lysosomal-associated protein transmembrane 4B 634 451 352 0.30
Genes with low expression
Mm.24678 Pard6g Par-6 partitioning defective 6 homolog gamma (C. elegans) 13 2 9 0.01
Mm.273155 2610110G12Rik RIKEN cDNA 2610110G12 gene 14 2 9 0.00
Mm.1249 Lamc1 Laminin, gamma 1 14 1 9 NA
Mm.261831    19 1 9 NA
Mm.257952 Tmem159 Transmembrane protein 159 20 2 9 0.01
  1. 1The 40 transcripts in common for oligoarrays, MPSS and SAGE among two subsets of 100 genes each, one with the highest and another with the lowest transcript concentrations. The values are listed as number of transcripts per cell for oligoarrays, MPSS and SAGE, and as relative to the endogenous control β-actin (Actb) for qRT-PCR. NA means not analysed.