Figure 5

Transcriptional responses of adult L. rubellus exposed to a series of concentrations of Cadmium. The array data used for this analysis were normalised per chip and per gene median polishing, expressed relative to the control samples; data from poor quantity spots were removed, and genes showing significant (p < 0.05) differential expression in response to Cadmium identified using ANOVA analysis with Benjamini and Hochberg False Discovery. Hierarchical clustering was performed using a distance algorithm for both conditions and genes. Panel A shows hierarchical clustering of the individual samples, whilst Panel B provides that hierarchical clustering of average expression generated by treatments. Panel C displays the average expression of a series of important functional groups pertinent to Cadmium toxicosis. Full details of the provenance of these reporters are provided in Additional File 10.