Figure 3

Real-time PCR validation of the microarray data, performed for 7 genes modulated by HCV. Total RNA from the 21-5 and 21-5 cured cell lines was used to assess mRNA levels using real time RT-PCR. Levels were normalized to cellular GAPDH; mRNA levels from 21-5 cured cells were set as the basis for the comparative results. Shown are the means (± SD) of three independent experiments. Fold-changes calculated for the microarray data are also indicated.