High-throughput gene and SNP discovery in Eucalyptus grandis, an uncharacterized genome

BMC Genomics

Table 1 Summary of E. grandis cDNA sequences.

Run number	Method	Platform	Number of reads	Average length	Total bp
1	454 pyrosequencing	GS-20	328,486	106.28 bp	34.9 Mbp
2	454 pyrosequencing	GS-20	303,149	102.54 bp	31.1 Mbp
3	454 pyrosequencing	GS-FLX	392,616	209.89 bp	82.4 Mbp
454 all (3 runs)	454 pyrosequencing	GS-20 + FLX	1,024,251	145.24 bp	148.4 Mbp
Sanger (control)	dideoxy sequencing	ABI 3100	86,328	522.18 bp	45.1 Mbp

Summary of the E. grandis expressed sequences generated with GS-20 and GS-FLX pyrosequencing runs, and control ESTs obtained from dideoxy-based sequencing of an analogous Eucalyptus library

ISSN: 1471-2164